Human AF4/FMR2 Family, Member 1 (AFF1) ELISA Kit | AFF1 elisa kit

Human AF4/FMR2 Family, Member 1 (AFF1) ELISA Kit

Cat. Num. AAA2022501

• Gene Names: AFF1; AF4; PBM1; MLLT2
• Reactivity: Human
• Synonyms: AF4/FMR2 Family; Member 1 (AFF1); Human AF4/FMR2 Family; Member 1 (AFF1) ELISA Kit; AF-4; AF4; AF4-MLL; MLL/AF4; MLLT2; PBM1; Myeloid/Lymphoid Or Mixed-Lineage Leukemia; Translocated To 2; Pre-B-Cell Monocytic Leukemia Partner 1; AFF1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of AF4/FMR2 Family, Member 1 (AFF1).; No significant cross-reactivity or interference between AF4/FMR2 Family, Member 1 (AFF1) and analogues was observed.
• Sequence Length: 1,211

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AF4/FMR2 Family, Member 1 (AFF1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AF4/FMR2 Family, Member 1 (AFF1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AFF1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to AF4/FMR2 Family, Member 1 (AFF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to AF4/FMR2 Family, Member 1 (AFF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AF4/FMR2 Family, Member 1 (AFF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AF4/FMR2 Family, Member 1 (AFF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for AFF1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 5174573
• NCBI GeneID: 4299
• NCBI Accession #: NP_005926.1
• NCBI GenBank Nucleotide #: NM_005935.2
• UniProt Accession #: P51825; B4DTU1; E9PBM3
• Molecular Weight: 131,578 Da
• NCBI Official Full Name: AF4/FMR2 family member 1 isoform 2
• NCBI Official Synonym Full Names: AF4/FMR2 family, member 1
• NCBI Official Symbol: AFF1
• NCBI Official Synonym Symbols: AF4; PBM1; MLLT2
• NCBI Protein Information: AF4/FMR2 family member 1
• UniProt Protein Name: AF4/FMR2 family member 1
• Protein Family: AF4/FMR2 family
• UniProt Gene Name: AFF1
• UniProt Synonym Gene Names: AF4; FEL; MLLT2; PBM1; Protein AF-4
• UniProt Entry Name: AFF1_HUMAN

NCBI Description

This gene encodes a member of the AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome family of proteins, which have been implicated in childhood lymphoblastic leukemia, Fragile X E site mental retardation, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

AF-4: A chromosomal aberration involving AFF1 is associated with acute leukemias. Translocation t(4;11)(q21;q23) with MLL/HRX. The result is a rogue activator protein. Belongs to the AF4 family.

Protein type: Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: transcription elongation factor complex

Molecular Function: protein binding; transcription factor activity

Biological Process: positive regulation of transcription, DNA-dependent