Human Adrenergic Receptor Beta 3 (ADRb3) ELISA Kit | ADRb3 elisa kit

Human Adrenergic Receptor Beta 3 (ADRb3) ELISA Kit

Cat. Num. AAA2705328

• Gene Names: ADRB3; BETA3AR
• Reactivity: Human
• Synonyms: Adrenergic Receptor Beta 3 (ADRb3); Human Adrenergic Receptor Beta 3 (ADRb3) ELISA Kit; BETA3AR; B3AR; Beta-3 adrenoreceptor; ADRb3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adrenergic Receptor Beta 3 (ADRb3).; No significant cross-reactivity or interference between Adrenergic Receptor Beta 3 (ADRb3) and analogues was observed.
• Sequence Length: 408

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.117ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenergic Receptor Beta 3 (ADRb3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenergic Receptor Beta 3 (ADRb3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADRb3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adrenergic Receptor Beta 3 (ADRb3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adrenergic Receptor Beta 3 (ADRb3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adrenergic Receptor Beta 3 (ADRb3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adrenergic Receptor Beta 3 (ADRb3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 40037473
• NCBI GeneID: 155
• Molecular Weight: 43,519 Da
• NCBI Official Full Name: adrenergic receptor beta 3
• NCBI Official Synonym Full Names: adrenoceptor beta 3
• NCBI Official Symbol: ADRB3
• NCBI Official Synonym Symbols: BETA3AR
• NCBI Protein Information: beta-3 adrenergic receptor
• UniProt Protein Name: Beta-3 adrenergic receptor
• Protein Family: Beta-3 adrenergic receptor
• UniProt Gene Name: ADRB3
• UniProt Synonym Gene Names: ADRB3R; B3AR; Beta-3 adrenoceptor

NCBI Description

The protein encoded by this gene belongs to the family of beta adrenergic receptors, which mediate catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor is located mainly in the adipose tissue and is involved in the regulation of lipolysis and thermogenesis. [provided by RefSeq, Feb 2009]

Uniprot Description

ADRB3: Beta-adrenergic receptors mediate the catecholamine- induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB3 sub-subfamily.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 8p11.23

Cellular Component: integral component of plasma membrane; nucleus; plasma membrane; receptor complex

Molecular Function: beta-3 adrenergic receptor binding; beta-adrenergic receptor activity; beta3-adrenergic receptor activity; epinephrine binding; norepinephrine binding; protein binding; protein homodimerization activity

Biological Process: adenylate cyclase activation; adenylate cyclase-activating adrenergic receptor signaling pathway; aging; arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; brown fat cell differentiation; carbohydrate metabolic process; cell-cell signaling; diet induced thermogenesis; eating behavior; energy reserve metabolic process; G-protein coupled receptor signaling pathway; G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger; G-protein signaling, coupled to cAMP nucleotide second messenger; generation of precursor metabolites and energy; heat generation; negative regulation of multicellular organism growth; norepinephrine-epinephrine vasodilation involved in regulation of systemic arterial blood pressure; positive regulation of MAPK cascade; receptor-mediated endocytosis; response to antibiotic; response to cold; thermoregulation

Disease: Obesity