SDS-Page
Adhesion Molecule With Ig Like Domain Protein 1 Recombinant Protein | AMIGO1 recombinant protein
Recombinant Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1)
Gene Names
Amigo1; ali2; Amigo
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
>90%
Synonyms
Adhesion Molecule With Ig Like Domain Protein 1; Recombinant Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1); ALI2; Alivin-2; Amphoterin-Induced Gene And Open Reading Frame; Amphoterin-induced protein 1; AMIGO1 recombinant protein
Host
E Coli
Purity/Purification
>90%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Sequence
GRA VVSCPAACLC ASNILSCSKQQLPNVPHSLP SYTALLDLSH NNLSRLRAEW TPTRLTQLHS LLLSHNHLNFISSEAFSPVP NLRYLDLSSN QLRTLDEFLF SDLQVLEVLL LYNNHIMAVDRCAFDDMAQL QKLYLSQNQI SRFPLELVKE GAKLPKLTLL DLSSNKLKNLPLPDLQKLPA WIKNGLYLHN NPLNCDCELY QLFSHWQYRQ LSSVMDFQEDLYCMNSKKLH NVFNLSFLNC GEYKERAWEA HLGDTLIIKC DTKQQGMTKVWVTPSNERVL DEVTNGTVSV SKDGSLLFQQ VQVEDGGVYT CYAMGETFNETLSVELKVHN FTLHGHHDTL NT
Sequence Length
492
Applicable Applications for AMIGO1 recombinant protein
SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
Predicted Molecular Mass
43.0kDa
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Endotoxin Level
<1.0EU per 1 ug (determined by the LAL method)
Expression System
Prokaryotic expression
Fragment
Gly28~Thr372
Organism Species
Human
Tag
two N-terminal Tags, His-tag and T7-tag
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
35,661 Da
NCBI Official Full Name
Adhesion molecule with Ig like domain 1
NCBI Official Synonym Full Names
adhesion molecule with Ig like domain 1
NCBI Official Symbol
Amigo1
NCBI Official Synonym Symbols
ali2; Amigo
NCBI Protein Information
amphoterin-induced protein 1
UniProt Protein Name
Amphoterin-induced protein 1
Protein Family
UniProt Gene Name
Amigo1
UniProt Entry Name
AMGO1_MOUSE
Uniprot Description
AMIGO1: Promotes growth and fasciculation of neurites from cultured hippocampal neurons. May be involved in fasciculation as well as myelination of developing neural axons. May have a role in regeneration as well as neural plasticity in the adult nervous system. May mediate homophilic as well as heterophilic cell-cell interaction and contribute to signal transduction through its intracellular domain. Belongs to the immunoglobulin superfamily. AMIGO family.
Protein type: Membrane protein, integral; Cell development/differentiation
Cellular Component: cell projection; dendrite; integral to membrane; integral to plasma membrane; membrane; plasma membrane; voltage-gated potassium channel complex
Molecular Function: potassium channel regulator activity; protein binding
Biological Process: axonal fasciculation; axonogenesis; cell adhesion; cell differentiation; heterophilic cell adhesion; homophilic cell adhesion; multicellular organismal development; myelination; nervous system development; positive regulation of axonogenesis; positive regulation of synaptogenesis
Research Articles on AMIGO1
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Product Notes
The AMIGO1 amigo1 (Catalog #AAA2033209) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Adhesion Molecule With Ig Like Domain Protein 1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the AMIGO1 amigo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GRA VVSCPAACLC ASNILSCSKQ QLPNVPHSLP SYTALLDLSH NNLSRLRAEW TPTRLTQLHS LLLSHNHLNF ISSEAFSPVP NLRYLDLSSN QLRTLDEFLF SDLQVLEVLL LYNNHIMAVD RCAFDDMAQL QKLYLSQNQI SRFPLELVKE GAKLPKLTLL DLSSNKLKNL PLPDLQKLPA WIKNGLYLHN NPLNCDCELY QLFSHWQYRQ LSSVMDFQED LYCMNSKKLH NVFNLSFLNC GEYKERAWEA HLGDTLIIKC DTKQQGMTKV WVTPSNERVL DEVTNGTVSV SKDGSLLFQQ VQVEDGGVYT CYAMGETFNE TLSVELKVHN FTLHGHHDTL NT. It is sometimes possible for the material contained within the vial of "Adhesion Molecule With Ig Like Domain Protein 1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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