Human Adenylate Cyclase 2, Brain (ADCY2) ELISA Kit | ADCY2 elisa kit

Human Adenylate Cyclase 2, Brain (ADCY2) ELISA Kit

Cat. Num. AAA2021480

• Gene Names: ADCY2; AC2; HBAC2
• Reactivity: Human
• Synonyms: Adenylate Cyclase 2; Brain (ADCY2); Human Adenylate Cyclase 2; Brain (ADCY2) ELISA Kit; HBAC2; ATP pyrophosphate-lyase 2; Adenylate cyclase type II; ADCY2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adenylate Cyclase 2, Brain (ADCY2).; No significant cross-reactivity or interference between Adenylate Cyclase 2, Brain (ADCY2) and analogues was observed.
• Sequence Length: 911

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.126ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenylate Cyclase 2, Brain (ADCY2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenylate Cyclase 2, Brain (ADCY2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADCY2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylate Cyclase 2, Brain (ADCY2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenylate Cyclase 2, Brain (ADCY2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylate Cyclase 2, Brain (ADCY2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenylate Cyclase 2, Brain (ADCY2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ADCY2 elisa kit

Enzyme & Kinase; Metabolic pathway; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 115387102
• NCBI GeneID: 108
• NCBI Accession #: NP_065433.2
• NCBI GenBank Nucleotide #: NM_020546.2
• UniProt Accession #: Q08462; Q2NKL8; Q9UDB2; Q9UPU2; B7Z2C1
• Molecular Weight: 103,158 Da
• NCBI Official Full Name: adenylate cyclase type 2
• NCBI Official Synonym Full Names: adenylate cyclase 2 (brain)
• NCBI Official Symbol: ADCY2
• NCBI Official Synonym Symbols: AC2; HBAC2
• NCBI Protein Information: adenylate cyclase type 2
• UniProt Protein Name: Adenylate cyclase type 2
• Protein Family: Adenylate cyclase
• UniProt Gene Name: ADCY2
• UniProt Synonym Gene Names: KIAA1060
• UniProt Entry Name: ADCY2_HUMAN

NCBI Description

This gene encodes a member of the family of adenylate cyclases, which are membrane-associated enzymes that catalyze the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). This enzyme is insensitive to Ca(2+)/calmodulin, and is stimulated by the G protein beta and gamma subunit complex. [provided by RefSeq, Jul 2008]

Uniprot Description

ADCY2: This is a membrane-bound, calmodulin-insensitive adenylyl cyclase. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.

Protein type: Membrane protein, integral; Nucleotide Metabolism - purine; EC 4.6.1.1; Transporter, aquaporin family; Adenylyl cyclase; Lyase; Membrane protein, multi-pass; Transporter

Chromosomal Location of Human Ortholog: 5p15.3

Cellular Component: protein complex; membrane; dendrite; cytoplasm; integral to membrane; plasma membrane; intracellular; lipid raft

Molecular Function: protein heterodimerization activity; metal ion binding; G-protein beta/gamma-subunit binding; calcium- and calmodulin-responsive adenylate cyclase activity; ATP binding; adenylate cyclase activity; adenylate cyclase binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; water transport; activation of protein kinase A; signal transduction; G-protein signaling, adenylate cyclase activating pathway; G-protein signaling, coupled to cAMP nucleotide second messenger; synaptic transmission; phospholipase C activation; energy reserve metabolic process; innate immune response; renal water homeostasis; cAMP biosynthetic process; G-protein signaling, adenylate cyclase inhibiting pathway; transmembrane transport