Typical Testing Data/Standard Curve (for reference only)
Human Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) ELISA Kit | ARPC2 elisa kit
Human Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) ELISA Kit
Gene Names
ARPC2; ARC34; PRO2446; p34-Arc; PNAS-139
Reactivity
Human
Synonyms
Actin Related Protein 2/3 Complex Subunit 2 (ARPC2); Human Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) ELISA Kit; ARC34; PNAS-139; p34-Arc; Arp2/3 complex 34 kDa subunit; ARPC2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of ARPC2. No significant cross-reactivity or interference between ARPC2 and analogues was observed.
Sequence Length
300
Samples
Tissue homogenates and other biological fluids.
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
0.068ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ARPC2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ARPC2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ARPC2 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ARPC2 in human tissue homogenates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ARPC2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ARPC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ARPC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ARPC2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ARPC2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ARPC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ARPC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ARPC2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,333 Da
NCBI Official Full Name
actin-related protein 2/3 complex subunit 2
NCBI Official Synonym Full Names
actin related protein 2/3 complex subunit 2
NCBI Official Symbol
ARPC2
NCBI Official Synonym Symbols
ARC34; PRO2446; p34-Arc; PNAS-139
NCBI Protein Information
actin-related protein 2/3 complex subunit 2
UniProt Protein Name
Actin-related protein 2/3 complex subunit 2
UniProt Gene Name
ARPC2
UniProt Synonym Gene Names
ARC34; p34-ARC
NCBI Description
This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.
Research Articles on ARPC2
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Product Notes
The Human ARPC2 arpc2 (Catalog #AAA454289) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA454289 ELISA Kit recognizes Human ARPC2. It is sometimes possible for the material contained within the vial of "Actin Related Protein 2/3 Complex Subunit 2 (ARPC2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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