Human Actin Like Protein 6A (ACTL6A) ELISA Kit | ACTL6A elisa kit

Human Actin Like Protein 6A (ACTL6A) ELISA Kit

Cat. Num. AAA2706840

• Gene Names: ACTL6A; Arp4; ACTL6; BAF53A; INO80K; ARPN-BETA
• Reactivity: Human
• Synonyms: Actin Like Protein 6A (ACTL6A); Human Actin Like Protein 6A (ACTL6A) ELISA Kit; Actl6; BAF53A; Arp4; Baf53a; INO80K; BAF Complex 53 kDa Subunit; 53 kDa BRG1-associated factor A; Actin-Related Protein 4; INO80 Complex Subunit K; ACTL6A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Actin Like Protein 6A (ACTL6A).; No significant cross-reactivity or interference between Actin Like Protein 6A (ACTL6A) and analogues was observed.
• Sequence Length: 387

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Actin Like Protein 6A (ACTL6A) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Actin Like Protein 6A (ACTL6A) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACTL6A elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actin Like Protein 6A (ACTL6A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Actin Like Protein 6A (ACTL6A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actin Like Protein 6A (ACTL6A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Actin Like Protein 6A (ACTL6A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 30089999
• NCBI GeneID: 86
• NCBI Accession #: NP_829888.1
• NCBI GenBank Nucleotide #: NM_178042.3
• UniProt Accession #: O96019; Q8TAE5; Q9BVS8; Q9H0W6; B3KMN1; D3DNR9
• Molecular Weight: 43,236 Da
• NCBI Official Full Name: actin-like protein 6A isoform 2
• NCBI Official Synonym Full Names: actin like 6A
• NCBI Official Symbol: ACTL6A
• NCBI Official Synonym Symbols: Arp4; ACTL6; BAF53A; INO80K; ARPN-BETA
• NCBI Protein Information: actin-like protein 6A
• UniProt Protein Name: Actin-like protein 6A
• Protein Family: Actin-like protein
• UniProt Gene Name: ACTL6A
• UniProt Synonym Gene Names: BAF53; BAF53A; INO80K; BAF53A

NCBI Description

This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

BAF53A: an actin-related protein involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for maximal ATPase activity of the helicase SMARCA4 and for association of the SMARCA4 containing remodelling complex BAF with chromatin/nuclear matrix. A component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of specific genes. Two alternatively spliced human isoforms have been described.

Chromosomal Location of Human Ortholog: 3q26.33

Cellular Component: NuA4 histone acetyltransferase complex; nuclear chromatin; nucleoplasm; nucleus; plasma membrane; protein complex; SWI/SNF complex

Molecular Function: chromatin binding; nucleosomal DNA binding; protein binding; transcription coactivator activity

Biological Process: ATP-dependent chromatin remodeling; chromatin remodeling; DNA recombination; DNA repair; protein deubiquitination; regulation of growth; regulation of transcription from RNA polymerase II promoter; signal transduction; spinal cord development; transcription, DNA-dependent