Typical Testing Data/Standard Curve (for reference only)
Human Acid sphingomyelinase like phosphodiesterase 3b (SMPDL3B) ELISA Kit | SMPDL3B elisa kit
Human Acid sphingomyelinase like phosphodiesterase 3b (SMPDL3B) ELISA Kit
Gene Names
SMPDL3B; ASML3B
Reactivity
Human
Synonyms
Acid sphingomyelinase like phosphodiesterase 3b (SMPDL3B); Human Acid sphingomyelinase like phosphodiesterase 3b (SMPDL3B) ELISA Kit; SMPDL3B elisa kit
Reactivity
Human
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SMPDL3B elisa kit
Intended Uses: This SMPDL3B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SMPDL3B. This ELISA kit for research use only!
Principle of the Assay: SMPDL3B ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SMPDL3B antibody and an SMPDL3B-HRP conjugate. The assay sample and buffer are incubated together with SMPDL3B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SMPDL3B concentration since SMPDL3B from samples and SMPDL3B-HRP conjugate compete for the anti-SMPDL3B antibody binding site. Since the number of sites is limited, as more sites are occupied by SMPDL3B from the sample, fewer sites are left to bind SMPDL3B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SMPDL3B concentration in each sample is interpolated from this standard curve.
Principle of the Assay: SMPDL3B ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SMPDL3B antibody and an SMPDL3B-HRP conjugate. The assay sample and buffer are incubated together with SMPDL3B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SMPDL3B concentration since SMPDL3B from samples and SMPDL3B-HRP conjugate compete for the anti-SMPDL3B antibody binding site. Since the number of sites is limited, as more sites are occupied by SMPDL3B from the sample, fewer sites are left to bind SMPDL3B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SMPDL3B concentration in each sample is interpolated from this standard curve.
Product Categories/Family for SMPDL3B elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,689 Da
NCBI Official Full Name
acid sphingomyelinase-like phosphodiesterase 3b isoform 1
NCBI Official Synonym Full Names
sphingomyelin phosphodiesterase, acid-like 3B
NCBI Official Symbol
SMPDL3B
NCBI Official Synonym Symbols
ASML3B
NCBI Protein Information
acid sphingomyelinase-like phosphodiesterase 3b; ASM-like phosphodiesterase 3b
UniProt Protein Name
Acid sphingomyelinase-like phosphodiesterase 3b
Protein Family
UniProt Gene Name
SMPDL3B
UniProt Synonym Gene Names
ASML3B; ASM-like phosphodiesterase 3b
UniProt Entry Name
ASM3B_HUMAN
Uniprot Description
SMPDL3B: Belongs to the acid sphingomyelinase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Phosphodiesterase; Secreted; Secreted, signal peptide; EC 3.1.4.-
Chromosomal Location of Human Ortholog: 1p35.3
Cellular Component: extracellular space
Molecular Function: sphingomyelin phosphodiesterase activity; hydrolase activity, acting on glycosyl bonds
Biological Process: sphingomyelin catabolic process
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Product Notes
The Human SMPDL3B smpdl3b (Catalog #AAA7242427) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7242427 ELISA Kit recognizes Human SMPDL3B. It is sometimes possible for the material contained within the vial of "Acid sphingomyelinase like phosphodiesterase 3b (SMPDL3B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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