Typical Testing Data/Standard Curve (for reference only)
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Human Acid Sphingomyelinase ELISA Kit | ASM elisa kit
Human Acid Sphingomyelinase ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: ASM ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ASM antibody and an ASM-HRP conjugate. The assay sample and buffer are incubated together with ASM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ASM concentration since ASM from samples and ASM-HRP conjugate compete for the anti-ASM antibody binding site. Since the number of sites is limited, as more sites are occupied by ASM from the sample, fewer sites are left to bind ASM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASM concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010]
Uniprot Description
SMPD1: Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity. Monomer. Belongs to the acid sphingomyelinase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Phosphodiesterase; Lipid Metabolism - sphingolipid; EC 3.1.4.12
Chromosomal Location of Human Ortholog: 11p15.4-p15.1
Cellular Component: extracellular space; lysosomal lumen; lysosome; plasma membrane; endosome; lamellar body
Molecular Function: protein binding; sphingomyelin phosphodiesterase activity; hydrolase activity, acting on glycosyl bonds
Biological Process: response to drug; negative regulation of MAP kinase activity; nervous system development; sphingolipid metabolic process; sphingomyelin metabolic process; positive regulation of apoptosis; ceramide biosynthetic process; glycosphingolipid metabolic process; sphingomyelin catabolic process; signal transduction; response to cocaine; positive regulation of protein amino acid dephosphorylation
Disease: Niemann-pick Disease, Type B; Niemann-pick Disease, Type A
Research Articles on ASM
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Product Notes
The Human ASM smpd1 (Catalog #AAA731118) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA731118 ELISA Kit recognizes Human ASM. It is sometimes possible for the material contained within the vial of "Acid Sphingomyelinase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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