Human acetyl-Coenzyme A carboxylase beta ELISA Kit | ACACB elisa kit

Human Acetyl-CoA carboxylase 2, ACACB ELISA Kit

Cat. Num. AAA2602872

• Gene Names: ACACB; ACC2; ACCB; HACC275
• Reactivity: Human
• Synonyms: acetyl-Coenzyme A carboxylase beta; Human Acetyl-CoA carboxylase 2; ACACB ELISA Kit; ACACB elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.

• Samples: Human Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Aav6 Concentration
• Assay Type: Sandwich
• Detection Range: 100 ng/ml-1.56 ng/ml
• Sensitivity: 0.5 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACACB elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human AAV6 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 134142062
• NCBI GeneID: 32
• NCBI Accession #: NP_001084.3
• NCBI GenBank Nucleotide #: NM_001093.3
• UniProt Accession #: O00763; Q16852; Q1HEC1; Q6KE87; Q6KE89; Q6TY48; A6NK36
• Molecular Weight: 276,541 Da
• NCBI Official Full Name: acetyl-CoA carboxylase 2
• NCBI Official Synonym Full Names: acetyl-CoA carboxylase beta
• NCBI Official Symbol: ACACB
• NCBI Official Synonym Symbols: ACC2; ACCB; HACC275
• NCBI Protein Information: acetyl-CoA carboxylase 2; ACC-beta; acetyl-Coenzyme A carboxylase beta
• UniProt Protein Name: Acetyl-CoA carboxylase 2
• UniProt Gene Name: ACACB
• UniProt Synonym Gene Names: ACC2; ACCB
• UniProt Entry Name: ACACB_HUMAN

NCBI Description

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. ACC-beta is thought to control fatty acid oxidation by means of the ability of malonyl-CoA to inhibit carnitine-palmitoyl-CoA transferase I, the rate-limiting step in fatty acid uptake and oxidation by mitochondria. ACC-beta may be involved in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. There is evidence for the presence of two ACC-beta isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

ACC2: a subunit of acetyl-CoA carboxylase (ACC), a multifunctional enzyme system. Catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. Activity is increased by oligomerization. Activated by citrate. Citrate and MID1IP1 promote oligomerization. Inhibited by malonyl-CoA. ACC1 is the predominant form expressed in liver, adipocyte and mammary gland. Phosphorylation by AMPK or PKA inhibits the enzymatic activity of ACC. ACC2 is the major isoform in skeletal muscle and heart. Phosphorylation regulates its activity. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Lipid Metabolism - fatty acid biosynthesis; EC 6.3.4.14; Ligase; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - propanoate; EC 6.4.1.2

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: mitochondrial outer membrane; mitochondrion; endomembrane system; nucleus; cytosol

Molecular Function: protein binding; acetyl-CoA carboxylase activity; metal ion binding; biotin carboxylase activity; biotin binding; ATP binding

Biological Process: response to drug; vitamin metabolic process; acetyl-CoA metabolic process; energy reserve metabolic process; positive regulation of cellular metabolic process; carnitine shuttle; cellular lipid metabolic process; response to organic cyclic substance; water-soluble vitamin metabolic process; biotin metabolic process; negative regulation of fatty acid oxidation; fatty acid biosynthetic process; protein homotetramerization