Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human 7SK snRNA methylphosphate capping enzyme (MEPCE) Standard Curve.)
Human 7SK snRNA Methylphosphate Capping Enzyme (MEPCE) ELISA Kit | MEPCE elisa kit
Human 7SK snRNA Methylphosphate Capping Enzyme (MEPCE) ELISA Kit
Gene Names
MEPCE; BCDIN3
Reactivity
Human
Synonyms
7SK snRNA Methylphosphate Capping Enzyme (MEPCE); Human 7SK snRNA Methylphosphate Capping Enzyme (MEPCE) ELISA Kit; MEPCE; BCDIN3; FLJ20257; 7SK snRNA methylphosphate capping enzyme; bin3; bicoid-interacting 3; homolog; MEPCE elisa kit
Reactivity
Human
Specificity
Human 7SK snRNA methylphosphate capping enzyme (MEPCE) ELISA Kit has high sensitivity and excellent specificity for detection of Human MEPCE. No significant cross-reactivity or interference between Human MEPCE and analogues was observed.
Sequence Length
689
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human 7SK snRNA methylphosphate capping enzyme (MEPCE) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human 7SK snRNA methylphosphate capping enzyme (MEPCE) Standard Curve.)
Related Product Information for MEPCE elisa kit
Description: This Human 7SK snRNA methylphosphate capping enzyme (MEPCE) ELISA Kit employs a two-site sandwich ELISA to quantitate MEPCE in samples. An antibody specific for MEPCE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MEPCE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MEPCE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MEPCE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MEPCE (Methylphosphate Capping Enzyme) is a Protein Coding gene. GO annotations related to this gene include poly(A) RNA binding and S-adenosylmethionine-dependent methyltransferase activity. S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5-end of 7SK snRNA, leading to stabilize it.
Background: MEPCE (Methylphosphate Capping Enzyme) is a Protein Coding gene. GO annotations related to this gene include poly(A) RNA binding and S-adenosylmethionine-dependent methyltransferase activity. S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5-end of 7SK snRNA, leading to stabilize it.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
7SK snRNA methylphosphate capping enzyme isoform A
NCBI Official Synonym Full Names
methylphosphate capping enzyme
NCBI Official Symbol
MEPCE
NCBI Official Synonym Symbols
BCDIN3
NCBI Protein Information
7SK snRNA methylphosphate capping enzyme
UniProt Protein Name
7SK snRNA methylphosphate capping enzyme
Protein Family
UniProt Gene Name
MEPCE
UniProt Synonym Gene Names
BCDIN3; MePCE; Bin3 homolog
UniProt Entry Name
MEPCE_HUMAN
Uniprot Description
BCDIN3: a CDK9-associated enzyme that forms the distinctive gamma-methylphosphate cap structure of 7SK, a noncoding RNA that regulates CDK9 activity. An adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to its stabilization. Component of the 7SK snRNP complex that contains P-TEFb (composed of CDK9 and CCNT1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis. Weakly or not expressed in other tissues. Contains a Bin3 domain found in a number of protein methyltransferases that modify RNA-binding proteins.
Protein type: RNA processing; Transcription regulation; EC 2.1.1.-; EC 2.1.1.; Methyltransferase
Chromosomal Location of Human Ortholog: 7q22.1
Molecular Function: S-adenosylmethionine-dependent methyltransferase activity; RNA methyltransferase activity
Biological Process: snRNA metabolic process; snRNA modification; RNA methylation; negative regulation of transcription from RNA polymerase II promoter
Research Articles on MEPCE
Similar Products
Product Notes
The Human MEPCE mepce (Catalog #AAA9715315) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715315 ELISA Kit recognizes Human MEPCE. It is sometimes possible for the material contained within the vial of "7SK snRNA Methylphosphate Capping Enzyme (MEPCE), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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