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Western Blot (WB) (MEPCE Antibody (Center) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the MEPCE antibody detected the MEPCE protein (arrow).)

Rabbit anti-Mouse MEPCE Polyclonal Antibody | anti-MEPCE antibody

MEPCE, ID (MEPCE, BCDIN3, 7SK snRNA methylphosphate capping enzyme, Bicoid-interacting protein 3 homolog) (Azide free) (HRP)

Gene Names
MEPCE; BCDIN3
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
MEPCE; Polyclonal Antibody; ID (MEPCE; BCDIN3; 7SK snRNA methylphosphate capping enzyme; Bicoid-interacting protein 3 homolog) (Azide free) (HRP); Bicoid-interacting protein 3 homolog; anti-MEPCE antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Sequence Length
220
Applicable Applications for anti-MEPCE antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
MEPCE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 238-267 amino acids from the Central region of human MEPCE.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MEPCE Antibody (Center) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the MEPCE antibody detected the MEPCE protein (arrow).)

Western Blot (WB) (MEPCE Antibody (Center) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the MEPCE antibody detected the MEPCE protein (arrow).)
Related Product Information for anti-MEPCE antibody
S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it.
Product Categories/Family for anti-MEPCE antibody
References
Xue, Y., et al. Nucleic Acids Res. 38(2):360-369(2010). Krueger, B.J., et al. Nucleic Acids Res. 36(7):2219-2229(2008). Kaneko, S., et al. Proc. Natl. Acad. Sci. U.S.A. 104(45):17620-17625(2007). Jeronimo, C., et al. Mol. Cell 27(2):262-274(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
7SK snRNA methylphosphate capping enzyme isoform B
NCBI Official Synonym Full Names
methylphosphate capping enzyme
NCBI Official Symbol
MEPCE
NCBI Official Synonym Symbols
BCDIN3
NCBI Protein Information
7SK snRNA methylphosphate capping enzyme
UniProt Protein Name
7SK snRNA methylphosphate capping enzyme
UniProt Gene Name
MEPCE
UniProt Synonym Gene Names
BCDIN3; MePCE; Bin3 homolog
UniProt Entry Name
MEPCE_HUMAN

Uniprot Description

BCDIN3: a CDK9-associated enzyme that forms the distinctive gamma-methylphosphate cap structure of 7SK, a noncoding RNA that regulates CDK9 activity. An adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to its stabilization. Component of the 7SK snRNP complex that contains P-TEFb (composed of CDK9 and CCNT1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis. Weakly or not expressed in other tissues. Contains a Bin3 domain found in a number of protein methyltransferases that modify RNA-binding proteins.

Protein type: RNA processing; Transcription regulation; EC 2.1.1.-; EC 2.1.1.; Methyltransferase

Chromosomal Location of Human Ortholog: 7q22.1

Molecular Function: S-adenosylmethionine-dependent methyltransferase activity; RNA methyltransferase activity

Biological Process: snRNA metabolic process; snRNA modification; RNA methylation; negative regulation of transcription from RNA polymerase II promoter

Research Articles on MEPCE

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Product Notes

The MEPCE mepce (Catalog #AAA6320258) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MEPCE, ID (MEPCE, BCDIN3, 7SK snRNA methylphosphate capping enzyme, Bicoid-interacting protein 3 homolog) (Azide free) (HRP) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MEPCE can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MEPCE mepce for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEPCE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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