Human 7-Dehydrocholesterol Reductase (DHCR7) ELISA Kit | DHCR7 elisa kit

Human 7-Dehydrocholesterol Reductase (DHCR7) ELISA Kit

Cat. Num. AAA2704323

• Gene Names: DHCR7; SLOS
• Reactivity: Human
• Synonyms: 7-Dehydrocholesterol Reductase (DHCR7); Human 7-Dehydrocholesterol Reductase (DHCR7) ELISA Kit; DHCR-7; SLOS; D7SR; Smith-Lemli-Opitz Syndrome; Putative sterol reductase SR-2; Sterol Delta(7)-reductase; DHCR7 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of 7-Dehydrocholesterol Reductase (DHCR7).; No significant cross-reactivity or interference between 7-Dehydrocholesterol Reductase (DHCR7) and analogues was observed.
• Sequence Length: 475

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 7-Dehydrocholesterol Reductase (DHCR7) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 7-Dehydrocholesterol Reductase (DHCR7) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Related Product Information for DHCR7 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 7-Dehydrocholesterol Reductase (DHCR7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 7-Dehydrocholesterol Reductase (DHCR7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 7-Dehydrocholesterol Reductase (DHCR7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 7-Dehydrocholesterol Reductase (DHCR7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 255308875
• NCBI GeneID: 1717
• NCBI Accession #: NP_001157289.1
• NCBI GenBank Nucleotide #: NM_001163817.1
• UniProt Accession #: Q9UBM7; O60492; O60717; B2R6Z2
• Molecular Weight: 54,489 Da
• NCBI Official Full Name: 7-dehydrocholesterol reductase
• NCBI Official Synonym Full Names: 7-dehydrocholesterol reductase
• NCBI Official Symbol: DHCR7
• NCBI Official Synonym Symbols: SLOS
• NCBI Protein Information: 7-dehydrocholesterol reductase
• UniProt Protein Name: 7-dehydrocholesterol reductase
• Protein Family: Probable 7-dehydrocholesterol reductase
• UniProt Gene Name: DHCR7
• UniProt Synonym Gene Names: D7SR; 7-DHC reductase

NCBI Description

This gene encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. This gene is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by cognitive disability, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Aug 2009]

Uniprot Description

Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC).