Rabbit anti-Human NFKBIL2 Polyclonal Antibody | anti-TONSL antibody

NFKBIL2 (TONSL, IKBR, Tonsoku-like Protein, Inhibitor of kappa B-related Protein, NF-kappa-B Inhibitor-like Protein 2, Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-cells Inhibitor-like 2, FLJ40087)

Cat. Num. AAA641440

• Gene Names: TONSL; IKBR; NFKBIL2
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: NFKBIL2; Polyclonal Antibody; NFKBIL2 (TONSL; IKBR; Tonsoku-like Protein; Inhibitor of kappa B-related Protein; NF-kappa-B Inhibitor-like Protein 2; Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-cells Inhibitor-like 2; FLJ40087); Anti -NFKBIL2 (TONSL; anti-TONSL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NFKBIL2.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MRTRLYLNLGLTFESLQQTALCNDYFRKSIFLAEQNHLYEDLFRARYNLGTIHWRAGQHSQAMRCLEGARECAHTMRKRFMESECCVVIAQVLQDLGDFLAAKRALKKAYRLGSQKPVQRAAICQNLQHVLAVVRLQQQLEEAEGRDPQGAMVICEQLGDLFSKAGDFPRAAEAYQKQLRFAELLDRPGAERAIIHVSLATTLGDMKDHHGAVRHYEEELRLRSGNVLEEAKTWLNIALSREEAGDAYELLAPCFQKALSCAQQAQRPQLQRQVLQHLHTVQLRLQPQEAPETETRLRELSVAEDEDEEEEAEEAAATAESEALEAGEVELSEGEDDTDGLTPQLEEDEELQGHLGRRKGSKWNRRNDMGETLLHRACIEGQLRRVQDLVRQGHPLNPRDYCGWTPLHEACNYGHLEIVRFLLDHGAAVDDPGGQGCEGITPLHDALNCGHFEVAELLLERGASVTLRTRKGLSPLETLQQWVKLYRRDLDLETRQKARAMEMLLQAAASGQDPHSSQAFHTPSSLLFDPETSPPLSPCPEPPSNSTRLPEASQVHVRVSPGQAAPAMARPRRSRHGPASSSSSSEGEDSAGPARPSQKRPRCSATAQRVAAWTPGPASNREAATASTSRAAYQAAIRGVGSAQSRLGPGPPRGHSKALAPQAALIPEEECLAGDWLELDMPLTRSRRPRPRGTGDNRRPSSTSGSDSEESRPRARAKQVRLTCMQSCSAPVNAGPSSLASEPPGSPSTPRVSEPSGDSSAAGQPLGPAPPPPIRVRVQVQDHLFLIPVPHSSDTHSVAWLAEQAAQRYYQTCGLLPRLTLRKEGALLAPQDLIPDVLQSNDEVLAEVTSWDLPPLTDRYRRACQSLGQGEHQQVLQAVELQGLGLSFSACSLALDQAQLTPLLRALKLHTALRELRLAGNRLGDKCVAELVAALGTMPSLALLDLSSNHLGPEGLRQLAMGLPGQATLQSLEELDLSMNPLGDGCGQSLASLLHACPLLSTLRLQACGFGPSFFLSHQTALGSAFQDAEHLKTLSLSYNALGAPALARTLQSLPAGTLLHLELSSVAAGKGDSDLMEPVFRYLAKEGCALAHLTLSANHLGDKAVRDLCRCLSLCPSLISLDLSANPEISCASLEELLSTLQKRPQGLSFLGLSGCAVQGPLGLGLWDKIAAQLRELQLCSRRLCAEDRDALRQLQPSRPGPGECTLDHGSKLFFRRL

• Applicable Applications for anti-TONSL antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full length human NFKBIL2, aa1-1219 (AAH08782.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of NFKBIL2 expression in transfected 293T cell line by NFKBIL2 polyclonal antibody. Lane 1: NFKBIL2 transfected lysate (132.9kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-TONSL antibody

The protein encoded by this gene is thought to be a negative regulator of NF-kappa-B mediated transcription. The encoded protein may bind NF-kappa-B complexes and trap them in the cytoplasm, preventing them from entering the nucleus and interacting with the DNA. Phosphorylation of this protein targets it for degradation by the ubiquitination pathway, which frees the NF-kappa-B complexes to enter the nucleus.

Product Categories/Family for anti-TONSL antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 119602499
• NCBI GeneID: 4796
• UniProt Accession #: Q96HA7; Q13006; Q9UGJ2; B5MDP0; C9JKB1; C9JNV8
• Molecular Weight: 150,929 Da
• NCBI Official Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 2, isoform CRA_b
• NCBI Official Synonym Full Names: tonsoku-like, DNA repair protein
• NCBI Official Symbol: TONSL
• NCBI Official Synonym Symbols: IKBR; NFKBIL2
• NCBI Protein Information: tonsoku-like protein; ikappaBR; I-kappa-B-related protein; NF-kappa-B inhibitor-like protein 2; inhibitor of kappa B-related protein; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 2
• UniProt Protein Name: Tonsoku-like protein
• UniProt Gene Name: TONSL
• UniProt Synonym Gene Names: IKBR; NFKBIL2; I-kappa-B-related protein; IkappaBR
• UniProt Entry Name: TONSL_HUMAN

NCBI Description

The protein encoded by this gene is thought to be a negative regulator of NF-kappa-B mediated transcription. The encoded protein may bind NF-kappa-B complexes and trap them in the cytoplasm, preventing them from entering the nucleus and interacting with the DNA. Phosphorylation of this protein targets it for degradation by the ubiquitination pathway, which frees the NF-kappa-B complexes to enter the nucleus. [provided by RefSeq, Jul 2008]

Uniprot Description

NFKBIL2: Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork- associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. Within the complex, may act as a scaffold. Belongs to the Tonsoku family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: nucleoplasm; DNA replication factor A complex; MCM complex; nuclear replication fork; cytoplasm

Molecular Function: protein binding; histone binding; transcription corepressor activity

Biological Process: cytoplasmic sequestering of transcription factor; double-strand break repair via homologous recombination; replication fork processing