Testing Data
(HEK-293 cells were transiently co-transfected with 500ng of human ST2-containing vector (or empty vector as a negative control), a vector containing NF-κB-driven firefly luciferase, and a vector containing Renilla luciferase as internal control in a 12-well plate. 40 hrs after transfection, cells were treated with recombinant human IL33 (His-tagged) followed by measurement with a dual luciferase assay.)
IL-33 Active Protein | IL33 active protein
IL-33, Recombinant, Human (His) (Interleukin-33, IL-1F11, NF-HEV)
>90% (SDS-PAGE)
>90% (SDS-PAGE)
Long-term Storage: -20 degree C
Working aliquots are stable for up to 3 months when stored at -20 degree C.
Testing Data
(HEK-293 cells were transiently co-transfected with 500ng of human ST2-containing vector (or empty vector as a negative control), a vector containing NF-κB-driven firefly luciferase, and a vector containing Renilla luciferase as internal control in a 12-well plate. 40 hrs after transfection, cells were treated with recombinant human IL33 (His-tagged) followed by measurement with a dual luciferase assay.)
Testing Data
(HEK-293 cells were transiently co-transfected with 500ng of human ST2-containing vector (or empty vector as a negative control), a vector containing NF-κB-driven firefly luciferase, and a vector containing Renilla luciferase as internal control in a 12-well plate. 40 hrs after transfection, cells were treated with recombinant human IL33 (His-tagged) followed by measurement with a dual luciferase assay.)
SDS-Page
(Pull down assay: a) pull down of hIL-33-His by hST2-Fc; b) pull down of hST2-Fc by hIL-33-His: 5ug of hST2-Fc (or control Fc protein), 2ug of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4°C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.)
SDS-Page
(Pull down assay: a) pull down of hIL-33-His by hST2-Fc; b) pull down of hST2-Fc by hIL-33-His: 5ug of hST2-Fc (or control Fc protein), 2ug of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4°C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.)
NCBI and Uniprot Product Information
NCBI Description
IL33 (MIM 608678) is a member of the IL1 (see MIM 147760) family that potently drives production of T helper-2 (Th2)-associated cytokines (e.g., IL4; MIM 147780). IL33 is a ligand for IL33R (IL1RL1; MIM 601203), an IL1 family receptor that is selectively expressed on Th2 cells and mast cells (summary by Yagami et al., 2010 [PubMed 20926795]).[supplied by OMIM, Jan 2011]
Uniprot Description
IL33: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Belongs to the IL-1 family. Highly divergent. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytokine
Chromosomal Location of Human Ortholog: 9p24.1
Cellular Component: extracellular space; transport vesicle; chromosome; nucleus
Molecular Function: protein binding; cytokine activity
Biological Process: positive regulation of T-helper 2 type immune response; negative regulation of T-helper 1 type immune response; T-helper 2 type immune response; transcription, DNA-dependent; positive regulation of interleukin-6 production; positive regulation of immunoglobulin secretion; negative regulation of leukocyte migration; positive regulation of interleukin-4 production; negative regulation of immunoglobulin secretion; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of interferon-gamma production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-13 production; positive regulation of interleukin-5 production; positive regulation of inflammatory response; positive regulation of macrophage activation
Research Articles on IL33
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Product Notes
The IL33 il33 (Catalog #AAA637686) is an Active Protein produced from Human, E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Human IL-33 (aa 112-270) is fused at the C-terminus to a His-tag. It is sometimes possible for the material contained within the vial of "IL-33, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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