Human Transmembrane Protein 27 (TMEM27) DIY ELISA Kit | TMEM27 diy elisa kit

Human Transmembrane Protein 27 (TMEM27) ELISA Kit DIY Materials

Cat. Num. AAA2089410

• Gene Names: TMEM27; NX17; NX-17
• Reactivity: Human
• Synonyms: Transmembrane Protein 27 (TMEM27); Human Transmembrane Protein 27 (TMEM27) ELISA Kit DIY Materials; NX17; Collectrin; TMEM27 diy elisa kit

• Reactivity: Human
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Transmembrane Protein 27 (TMEM27). No significant cross-reactivity or interference between Transmembrane Protein 27 (TMEM27) and analogues was observed.
• Sequence Length: 222

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.056ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for TMEM27 diy elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 119619300
• NCBI GeneID: 57393
• Molecular Weight: 25,235 Da
• NCBI Official Full Name: transmembrane protein 27
• NCBI Official Synonym Full Names: transmembrane protein 27
• NCBI Official Symbol: TMEM27
• NCBI Official Synonym Symbols: NX17; NX-17
• NCBI Protein Information: collectrin
• UniProt Protein Name: Collectrin
• Protein Family: Collectrin
• UniProt Gene Name: TMEM27

NCBI Description

This gene encodes a type 1 transmembrane protein that is important for trafficking amino acid transporters to the apical brush border of proximal tubules. The encoded protein binds to amino acid transporters and regulates their expression on the plasma membrane. It also plays a role in controlling insulin exocytosis by regulating formation of the SNARE (soluble N-ethylmaleimide-sensitive-factor attachment protein receptor) complex in pancreatic beta cells. The extracellular domain of the encoded protein may be cleaved and shed from the plasma membrane specifically in pancreatic beta cells. [provided by RefSeq, Jun 2013]

Uniprot Description

TMEM27: Regulator of SNARE complex function. Stimulator of beta cell replication. Belongs to the TMEM27 family.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.2

Cellular Component: cytoplasm

Molecular Function: protein binding

Biological Process: positive regulation of calcium ion-dependent exocytosis