Human Tight Junction Protein 1 (TJP1) DIY ELISA Kit | TJP1 diy elisa kit

Human Tight Junction Protein 1 (TJP1) ELISA Kit DIY Materials

Cat. Num. AAA2089261

• Gene Names: TJP1; ZO-1
• Reactivity: Human
• Synonyms: Tight Junction Protein 1 (TJP1); Human Tight Junction Protein 1 (TJP1) ELISA Kit DIY Materials; ZO1; Zona Occludens 1; Zona occludens protein 1; Zonula occludens protein 1; TJP1 diy elisa kit

• Reactivity: Human
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Tight Junction Protein 1 (TJP1). No significant cross-reactivity or interference between Tight Junction Protein 1 (TJP1) and analogues was observed.
• Sequence Length: 1748

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.059ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for TJP1 diy elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 116875767
• NCBI GeneID: 7082
• NCBI Accession #: NP_003248.3
• NCBI GenBank Nucleotide #: NM_003257.4
• UniProt Accession #: Q07157; Q2NKP3; Q4ZGJ6; B4E3K1
• Molecular Weight: 186,966 Da
• NCBI Official Full Name: tight junction protein ZO-1 isoform a
• NCBI Official Synonym Full Names: tight junction protein 1
• NCBI Official Symbol: TJP1
• NCBI Official Synonym Symbols: ZO-1
• NCBI Protein Information: tight junction protein ZO-1
• UniProt Protein Name: Tight junction protein ZO-1
• Protein Family: Tight junction protein
• UniProt Gene Name: TJP1
• UniProt Synonym Gene Names: ZO1

NCBI Description

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

ZO1: a scaffolding protein of the MAGUK (membrane-associated guanylate kinase) family associated with tight junctions (TJ). May mediate dynamic changes in the composition of TJs. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting the protein kinase MRCKb to the leading edge of migrating cells. Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts with HSPA4 and KIRREL1. Forms homodimers and heterodimers with ZO-2 or ZO-3. Interacts with occludin, CALM, claudins, CGN, CXADR, GJA12, GJD3 and UBN1. Interacts (via ZU5 domain) with MRCKb and MYZAP. Interacts (via PDZ domain) with Cx43. ZO-2 and ZO-3 shuttle between the TJ and the nucleus, where they may regulate gene expression. TJ, or zonula occludens, are the closely associated areas of two cells whose membranes are tightly joined together. They are composed of a branching network of strands, the efficiency of which increases exponentially with the number of strands. The strands associate with transmembrane proteins that bind to similar proteins on adjacent cells, and with submembranous proteins that are anchored to the actin component of the cytoskeleton. Thus, TJs join together the cytoskeletons of adjacent cells. They endow tissues with substantial form, shape and location, enforce cellular polarity, and form barriers to molecules and pathogens. Molecules forming the membranous part of TJs include occludin, claudins, tricellulin and junctional adhesion molecules. These molecules interact with the proteins ZO-1, ZO-2 and ZO-3. Tight junction proteins can be up- or downregulated in cancer and are involved in the epithelial-mesenchymal transitions (EMT) in tumors. Alternative splicing produces two isoforms. The long isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q13.1

Cellular Component: apical junction complex; apical part of cell; basolateral plasma membrane; cell junction; cell-cell adherens junction; cytoplasm; cytosol; plasma membrane; tight junction

Molecular Function: protein binding

Biological Process: intercellular junction assembly