Human Synaptophysin (SYP) DIY ELISA Kit | SYP diy elisa kit

Human Synaptophysin (SYP) ELISA Kit DIY Materials

Cat. Num. AAA2088556

• Gene Names: SYP; MRX96; MRXSYP
• Reactivity: Human
• Synonyms: Synaptophysin (SYP); Human Synaptophysin (SYP) ELISA Kit DIY Materials; Major synaptic vesicle protein p38; SYP diy elisa kit

• Reactivity: Human
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Synaptophysin (SYP). No significant cross-reactivity or interference between Synaptophysin (SYP) and analogues was observed.
• Sequence Length: 313

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.117ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for SYP diy elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 27764867
• NCBI GeneID: 6855
• NCBI Accession #: NP_003170.1
• NCBI GenBank Nucleotide #: NM_003179.2
• UniProt Accession #: P08247; Q6P2F7; B2R7L6; B7Z359
• Molecular Weight: 20,757 Da
• NCBI Official Full Name: synaptophysin
• NCBI Official Synonym Full Names: synaptophysin
• NCBI Official Symbol: SYP
• NCBI Official Synonym Symbols: MRX96; MRXSYP
• NCBI Protein Information: synaptophysin
• UniProt Protein Name: Synaptophysin
• Protein Family: Synaptophysin
• UniProt Gene Name: SYP

NCBI Description

This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR). [provided by RefSeq, Aug 2011]

Uniprot Description

SYP: Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. Homohexamer or homotetramer. Interacts with SRCIN1. Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. Belongs to the synaptophysin/synaptobrevin family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Vesicle

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: integral to synaptic vesicle membrane; neuron projection; synaptic vesicle

Molecular Function: cholesterol binding; protein self-association

Biological Process: endocytosis; regulation of long-term neuronal synaptic plasticity; regulation of neuronal synaptic plasticity; regulation of short-term neuronal synaptic plasticity

Disease: Mental Retardation, X-linked 96