Human Peroxisome Proliferator Activated Receptor Alpha (PPARa) DIY ELISA Kit | PPARa diy elisa kit
Human Peroxisome Proliferator Activated Receptor Alpha (PPARa) ELISA Kit DIY Materials
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
NCBI and Uniprot Product Information
NCBI Description
Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
PPAR-alpha: Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. Belongs to the nuclear hormone receptor family. NR1 subfamily.
Protein type: DNA-binding; Nuclear receptor
Chromosomal Location of Human Ortholog: 22q13.31
Cellular Component: nucleoplasm; nucleus
Molecular Function: DNA binding; drug binding; ligand-dependent nuclear receptor activity; lipid binding; protein binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription factor activity; transcription factor binding; ubiquitin conjugating enzyme binding
Biological Process: cellular lipid metabolic process; circadian regulation of gene expression; fatty acid transport; lipid metabolic process; negative regulation of appetite; negative regulation of glycolysis; negative regulation of inflammatory response; negative regulation of transcription from RNA polymerase II promoter; positive regulation of fatty acid beta-oxidation; positive regulation of fatty acid oxidation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of circadian rhythm; transcription initiation from RNA polymerase II promoter
Research Articles on PPARa
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Product Notes
The Human PPARa ppara (Catalog #AAA2088942) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2088942 DIY ELISA Kit recognizes Human PPARa. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the PPARa ppara for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Peroxisome Proliferator Activated Receptor Alpha (PPARa), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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