Human Peroxisome Proliferator Activated Receptor Alpha ELISA Kit | PPAR-alpha elisa kit

Human Peroxisome Proliferator Activated Receptor Alpha ELISA Kit

Cat. Num. AAA765329

• Gene Names: PPARA; PPAR; NR1C1; hPPAR; PPARalpha
• Reactivity: Human
• Synonyms: Peroxisome Proliferator Activated Receptor Alpha; Human Peroxisome Proliferator Activated Receptor Alpha ELISA Kit; PPAR-alpha ; PPAR-alpha elisa kit

• Reactivity: Human
• Sequence Length: 468

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PPAR-alpha elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human PPAR-alpha antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PPAR-alpha antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PPAR-alpha amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PPAR-alpha can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 765240
• NCBI GeneID: 5465
• Molecular Weight: 18,942 Da
• NCBI Official Full Name: peroxisome proliferator activated receptor alpha
• NCBI Official Synonym Full Names: peroxisome proliferator activated receptor alpha
• NCBI Official Symbol: PPARA
• NCBI Official Synonym Symbols: PPAR; NR1C1; hPPAR; PPARalpha
• NCBI Protein Information: peroxisome proliferator-activated receptor alpha
• UniProt Protein Name: Peroxisome proliferator-activated receptor alpha
• UniProt Gene Name: PPARA
• UniProt Synonym Gene Names: NR1C1; PPAR; PPAR-alpha
• UniProt Entry Name: PPARA_HUMAN

NCBI Description

Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

PPAR-alpha: Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. Belongs to the nuclear hormone receptor family. NR1 subfamily.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: nucleoplasm; nucleus

Molecular Function: DNA binding; drug binding; ligand-dependent nuclear receptor activity; lipid binding; NFAT protein binding; phosphatase binding; protein binding; protein complex binding; protein domain specific binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription factor activity; ubiquitin conjugating enzyme binding; zinc ion binding

Biological Process: behavioral response to nicotine; cellular lipid metabolic process; circadian regulation of gene expression; circadian rhythm; epidermis development; fatty acid metabolic process; fatty acid transport; gene expression; heart development; intracellular receptor-mediated signaling pathway; lipid metabolic process; lipoprotein metabolic process; negative regulation of appetite; negative regulation of blood pressure; negative regulation of glycolysis; negative regulation of inflammatory response; negative regulation of protein binding; negative regulation of transcription from RNA polymerase II promoter; positive regulation of fatty acid beta-oxidation; positive regulation of fatty acid oxidation; positive regulation of gluconeogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of circadian rhythm; response to hypoxia; response to insulin stimulus; steroid hormone mediated signaling; transcription initiation from RNA polymerase II promoter; wound healing