Human Inter Alpha-Globulin Inhibitor H2 (ITIH2) DIY ELISA Kit | ITIH2 diy elisa kit

Human Inter Alpha-Globulin Inhibitor H2 (ITIH2) ELISA Kit DIY Materials

Cat. Num. AAA2089395

• Gene Names: ITIH2; H2P; SHAP
• Reactivity: Human
• Synonyms: Inter Alpha-Globulin Inhibitor H2 (ITIH2); Human Inter Alpha-Globulin Inhibitor H2 (ITIH2) ELISA Kit DIY Materials; H2P; SHAP; IGHEP2; Serum-derived hyaluronan-associated protein; Inter-alpha-trypsin inhibitor complex component II; ITIH2 diy elisa kit

• Reactivity: Human
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Inter Alpha-Globulin Inhibitor H2 (ITIH2). No significant cross-reactivity or interference between Inter Alpha-Globulin Inhibitor H2 (ITIH2) and analogues was observed.
• Sequence Length: 946

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Detection Range: 3.12-200ng/mL
• Sensitivity: 1.42ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for ITIH2 diy elisa kit

Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 70778918
• NCBI GeneID: 3698
• NCBI Accession #: NP_002207.2
• NCBI GenBank Nucleotide #: NM_002216.2
• UniProt Accession #: P19823; Q14659; Q15484; Q5T986
• Molecular Weight: 106,463 Da
• NCBI Official Full Name: inter-alpha-trypsin inhibitor heavy chain H2
• NCBI Official Synonym Full Names: inter-alpha-trypsin inhibitor heavy chain 2
• NCBI Official Symbol: ITIH2
• NCBI Official Synonym Symbols: H2P; SHAP
• NCBI Protein Information: inter-alpha-trypsin inhibitor heavy chain H2
• UniProt Protein Name: Inter-alpha-trypsin inhibitor heavy chain H2
• Protein Family: Inter-alpha-trypsin inhibitor
• UniProt Gene Name: ITIH2
• UniProt Synonym Gene Names: IGHEP2; ITI heavy chain H2; ITI-HC2; Inter-alpha-inhibitor heavy chain 2; SHAP

NCBI Description

The inter-alpha-trypsin inhibitors (ITI) are a family of structurally related plasma serine protease inhibitors involved in extracellular matrix stabilization and in prevention of tumor metastasis. The ITI family contains multiple proteins made up of a light chain (see MIM 176870) and a variable number of heavy chains (Salier et al., 1987 [PubMed 2446322]; Himmelfarb et al., 2004 [PubMed 14744536]).[supplied by OMIM, Nov 2009]

Uniprot Description

ITIH2: May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes. Belongs to the ITIH family.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10p14

Molecular Function: endopeptidase inhibitor activity