Human Growth Differentiation Factor 2 (GDF2) DIY ELISA Kit | GDF2 diy elisa kit

Human Growth Differentiation Factor 2 (GDF2) ELISA Kit DIY Materials

Cat. Num. AAA2089147

• Gene Names: GDF2; BMP9; HHT5; BMP-9
• Reactivity: Human
• Synonyms: Growth Differentiation Factor 2 (GDF2); Human Growth Differentiation Factor 2 (GDF2) ELISA Kit DIY Materials; BMP9; Bone Morphogenetic Protein 9; GDF2 diy elisa kit

• Reactivity: Human
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Growth Differentiation Factor 2 (GDF2). No significant cross-reactivity or interference between Growth Differentiation Factor 2 (GDF2) and analogues was observed.
• Sequence Length: 429

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Detection Range: 62.5-4000pg/mL
• Sensitivity: 26.3pg/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for GDF2 diy elisa kit

Cytokine; Metabolic pathway; Developmental science; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 119571045
• NCBI GeneID: 2658
• Molecular Weight: 47,320 Da
• NCBI Official Full Name: growth differentiation factor 2
• NCBI Official Synonym Full Names: growth differentiation factor 2
• NCBI Official Symbol: GDF2
• NCBI Official Synonym Symbols: BMP9; HHT5; BMP-9
• NCBI Protein Information: growth/differentiation factor 2
• UniProt Protein Name: Growth/differentiation factor 2
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: GDF2
• UniProt Synonym Gene Names: BMP9; GDF-2; BMP-9

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates cartilage and bone development, angiogenesis and differentiation of cholinergic central nervous system neurons. Mutations in this gene are associated with hereditary hemorrhagic telangiectasia. [provided by RefSeq, Jul 2016]

Uniprot Description

GDF2: Could be involved in bone formation. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q11.22

Cellular Component: extracellular space

Molecular Function: cytokine activity; protein binding; transforming growth factor beta receptor binding

Biological Process: activin receptor signaling pathway; angiogenesis; blood vessel morphogenesis; BMP signaling pathway; cartilage development; cell development; cellular iron ion homeostasis; negative regulation of angiogenesis; negative regulation of blood vessel endothelial cell migration; negative regulation of cell growth; negative regulation of DNA replication; negative regulation of endothelial cell proliferation; ossification; patterning of blood vessels; positive regulation of angiogenesis; positive regulation of endothelial cell proliferation; positive regulation of interleukin-8 production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of apoptosis; regulation of MAPKKK cascade

Disease: Telangiectasia, Hereditary Hemorrhagic, Type 5