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Human Gastric Inhibitory Polypeptide (GIP) DIY ELISA Kit | GIP diy elisa kit

Human Gastric Inhibitory Polypeptide (GIP) ELISA Kit DIY Materials

Reactivity
Human
Synonyms
Gastric Inhibitory Polypeptide (GIP); Human Gastric Inhibitory Polypeptide (GIP) ELISA Kit DIY Materials; Incretin hormone; Glucose Dependent Insulinotropic Peptide; GIP diy elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Gastric Inhibitory Polypeptide (GIP). No significant cross-reactivity or interference between Gastric Inhibitory Polypeptide (GIP) and analogues was observed.
Sequence Length
153
Application Notes
Main materials for "Do It Yourself ELISA Kit"
Assay Type
Competitive Inhibition ELISA for Antigen Detection
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range
61.7-5000pg/mL
Sensitivity
23.9pg/mL
Reagent Contents
Capture Antibody, Biontin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 2. (Catalog MBS2089472)
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
Product Categories/Family for GIP diy elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,108 Da
NCBI Official Full Name
gastric inhibitory polypeptide preproprotein
NCBI Official Synonym Full Names
gastric inhibitory polypeptide
NCBI Official Symbol
GIP
NCBI Protein Information
gastric inhibitory polypeptide
UniProt Protein Name
Gastric inhibitory polypeptide
UniProt Gene Name
GIP
UniProt Synonym Gene Names
GIP

NCBI Description

This gene encodes an incretin hormone and belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption. This gene stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion. [provided by RefSeq, Jul 2008]

Uniprot Description

GIP: Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion. Belongs to the glucagon family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: endoplasmic reticulum lumen; extracellular region

Molecular Function: protein binding

Biological Process: regulation of insulin secretion; signal transduction

Research Articles on GIP

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Product Notes

The Human GIP gip (Catalog #AAA2088902) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2088902 DIY ELISA Kit recognizes Human GIP. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the GIP gip for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Gastric Inhibitory Polypeptide (GIP), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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