Human Corticotropin Releasing Hormone (CRH) DIY ELISA Kit | CRH diy elisa kit

Human Corticotropin Releasing Hormone (CRH) ELISA Kit DIY Materials

Cat. Num. AAA2088852

• Gene Names: CRH; CRF; CRH1
• Reactivity: Human
• Synonyms: Corticotropin Releasing Hormone (CRH); Human Corticotropin Releasing Hormone (CRH) ELISA Kit DIY Materials; CRF; Corticotropin Releasing Factor; Corticoliberin; CRH diy elisa kit

• Reactivity: Human
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Corticotropin Releasing Hormone (CRH). No significant cross-reactivity or interference between Corticotropin Releasing Hormone (CRH) and analogues was observed.
• Sequence Length: 196

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Competitive Inhibition ELISA for Antigen Detection
• Samples: Serum, plasma and other biological fluids.
• Detection Range: 12.35-1000pg/mL
• Sensitivity: 4.91pg/mL
• Reagent Contents: Capture Antibody, Biontin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 2. (Catalog MBS2089472)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for CRH diy elisa kit

Endocrinology; Cardiovascular biology; Neuroscience; Hormone metabolism; Urology

NCBI and Uniprot Product Information

• NCBI GI #: 119607303
• NCBI GeneID: 1392
• Molecular Weight: 21,422 Da
• NCBI Official Full Name: corticotropin releasing hormone
• NCBI Official Synonym Full Names: corticotropin releasing hormone
• NCBI Official Symbol: CRH
• NCBI Official Synonym Symbols: CRF; CRH1
• NCBI Protein Information: corticoliberin
• UniProt Protein Name: Corticoliberin
• Protein Family: Corticoliberin
• UniProt Gene Name: CRH
• UniProt Synonym Gene Names: CRF

NCBI Description

This gene encodes a member of the corticotropin-releasing factor family. The encoded preproprotein is proteolytically processed to generate the mature neuropeptide hormone. In response to stress, this hormone is secreted by the paraventricular nucleus (PVN) of the hypothalamus, binds to corticotropin releasing hormone receptors and stimulates the release of adrenocorticotropic hormone from the pituitary gland. Marked reduction in this protein has been observed in association with Alzheimer's disease. Autosomal recessive hypothalamic corticotropin deficiency has multiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes, and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturition. [provided by RefSeq, Nov 2015]

Uniprot Description

CRH: This hormone from hypothalamus regulates the release of corticotropin from pituitary gland. Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8q13.1

Cellular Component: extracellular region; extracellular space

Molecular Function: adrenocorticotropin-releasing hormone activity; hormone activity; neuropeptide hormone activity; protein binding; receptor binding

Biological Process: female pregnancy; inflammatory response; learning and/or memory; negative regulation of epinephrine secretion; parturition; positive regulation of cortisol secretion; signal transduction; synaptic transmission; synaptic transmission, dopaminergic

Disease: Acth Deficiency, Isolated