Human Atrial Natriuretic Peptide (ANP) DIY ELISA Kit | ANP diy elisa kit
Human Atrial Natriuretic Peptide (ANP) ELISA Kit DIY Materials
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. This gene is located adjacent to another member of the natriuretic family of peptides on chromosome 1. [provided by RefSeq, Oct 2015]
Uniprot Description
NPPA: Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Defects in NPPA are the cause of familial atrial fibrillation type 6 (ATFB6). Atrial fibrillation is a common disorder of cardiac rhythm that is hereditary in a small subgroup of patients. It is characterized by disorganized atrial electrical activity, progressive deterioration of atrial electromechanical function and ineffective pumping of blood into the ventricles. It can be associated with palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the natriuretic peptide family.
Protein type: Hormone; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1p36.22
Cellular Component: extracellular region
Molecular Function: neuropeptide hormone activity; protein binding; receptor binding
Biological Process: cellular protein metabolic process; cGMP biosynthetic process; female pregnancy; neuropeptide signaling pathway; positive regulation of heart rate; receptor guanylyl cyclase signaling pathway; regulation of blood pressure; transcription initiation from RNA polymerase II promoter
Disease: Atrial Fibrillation, Familial, 6; Atrial Standstill 2
Research Articles on ANP
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Product Notes
The Human ANP nppa (Catalog #AAA2088479) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2088479 DIY ELISA Kit recognizes Human ANP. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the ANP nppa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Atrial Natriuretic Peptide (ANP), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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