Human Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) CLIA Kit | WNT5B clia kit

Human Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) CLIA Kit

Cat. Num. AAA2000578

• Reactivity: Human
• Synonyms: Wingless Type MMTV Integration Site Family; Member 5B (WNT5B); Human Wingless Type MMTV Integration Site Family; Member 5B (WNT5B) CLIA Kit; WNT5B clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 5B (WNT5B).; No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) and analogues was observed.
• Sequence Length: 359

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.14-100ng/mL
• Sensitivity: < 0.05ng/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WNT5B clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 5B (WNT5B). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 5B (WNT5B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) level in the sample or standard.;

Product Categories/Family for WNT5B clia kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 12804649
• NCBI GeneID: 81029
• UniProt Accession #: Q9H1J7; Q96S49; Q9BV04; A8K315; D3DUP9
• Molecular Weight: 40,323 Da
• NCBI Official Full Name: Wingless-type MMTV integration site family, member 5B
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 5B
• NCBI Official Symbol: WNT5B
• NCBI Protein Information: protein Wnt-5b
• UniProt Protein Name: Protein Wnt-5b
• UniProt Gene Name: WNT5B
• UniProt Entry Name: WNT5B_HUMAN

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

WNT5B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Interacts with PORCN. Belongs to the Wnt family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: extracellular space; proteinaceous extracellular matrix; cell surface; Golgi lumen; endoplasmic reticulum lumen; extracellular region; plasma membrane

Molecular Function: frizzled binding; receptor binding

Biological Process: fat cell differentiation; neuron differentiation; cell fate commitment; Wnt receptor signaling pathway; wound healing; positive regulation of fat cell differentiation; chondrocyte differentiation; positive regulation of cell migration