Human Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) CLIA Kit | Rac1 clia kit

Human Ras Related C3 Botulinum Toxin Substrate 1 (Rac1)(CLIA) CLIA Kit

Cat. Num. AAA2709734

• Gene Names: RAC1; MIG5; Rac-1; TC-25; p21-Rac1
• Reactivity: Human
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Ras Related C3 Botulinum Toxin Substrate 1 (Rac1); Human Ras Related C3 Botulinum Toxin Substrate 1 (Rac1)(CLIA) CLIA Kit; CLIA Kit for Ras Related C3 Botulinum Toxin Substrate 1 (Rac1); Rac1/Ras Related C3 Botulinum Toxin Substrate 1; TC-25; p21-Rac1; MIG5; Cell migration-inducing gene 5 protein; Ras-like protein TC25; Rac1 clia kit

• Reactivity: Human
• Specificity: Reactive with Human Ras Related C3 Botulinum Toxin Substrate 1 (Rac1); This assay has high sensitivity and excellent specificity for detection of Ras Related C3 Botulinum Toxin Substrate 1 (Rac1). No significant cross-reactivity or interference between Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) and analogues was observed.

• Applicable Applications for Rac1 clia kit: Chemiluminescence Immunoassay (CLIA)
• Application Notes: Chemiluminescent immunoassay for Antigen Detection.
• Assay Type: Sandwich CLIA
• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Detection Method: Double-antibody Sandwich
• Detection Range: 13.7-10,000pg/mL
• Sensitivity: 5.1pg/mL
• Assay Length: 2.6 h
• Sample Size: 100ul (Please refer to the manual for the specific data.)
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Assay Procedure Summary: 1. Prepare all reagents, samples and standards; 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C; 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C; 4. Aspirate and wash 3 times; 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C; 6. Aspirate and wash 5 times; 7. Add 100ul Substrate Solution. Incubate 10 minutes at 37 degree C; 8. Read RLU value immediately.
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to X-Ray Repair Cross Complementing 6 (XRCC6). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to X-Ray Repair Cross Complementing 6 (XRCC6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the X-Ray Repair Cross Complementing 6 (XRCC6) level in the sample or standard.
• Preparation and Storage: Store at 4 degree C for 1 month/ -20 degree C for long-term (within shelf life).; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Product Categories/Family for Rac1 clia kit

Signal transduction; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 51702787
• NCBI GeneID: 5879
• UniProt Accession #: P63000; O95501; P15154; Q3Y4D3; Q5JAA8; Q9BTB4; AAB22206
• Molecular Weight: 192
• NCBI Official Full Name: Ras-related C3 botulinum toxin substrate 1
• NCBI Official Synonym Full Names: ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
• NCBI Official Symbol: RAC1
• NCBI Official Synonym Symbols: MIG5; Rac-1; TC-25; p21-Rac1
• NCBI Protein Information: ras-related C3 botulinum toxin substrate 1; ras-like protein TC25; cell migration-inducing gene 5 protein
• UniProt Protein Name: Ras-related C3 botulinum toxin substrate 1
• Protein Family: Ras-related protein
• UniProt Gene Name: RAC1
• UniProt Synonym Gene Names: TC25
• UniProt Entry Name: RAC1_HUMAN

NCBI Description

The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

RAC1: a plasma membrane-associated member of the Rho-GTPase family. Plays a key role in cytoskeletal reorganization, membrane trafficking, transcriptional regulation and cell growth and development. GTP binding stimulates its activity. Phosphorylation by Akt may inhibit GTP binding of Rac1, therefore attenuating the downstream signal transduction pathway. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; G protein; G protein, monomeric, Rho; G protein, monomeric

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: extrinsic to plasma membrane; Golgi membrane; focal adhesion; membrane; lamellipodium; cytoplasm; plasma membrane; melanosome; actin filament; trans-Golgi network; cytosol; phagocytic cup

Molecular Function: GTPase activity; protein binding; enzyme binding; GTP binding; GTP-dependent protein binding; Rho GDP-dissociation inhibitor binding; thioesterase binding; protein kinase binding; Rab GTPase binding

Biological Process: viral reproduction; nerve growth factor receptor signaling pathway; metabolic process; positive regulation of apoptosis; cell motility involved in cell locomotion; regulation of cell migration; small GTPase mediated signal transduction; positive regulation of stress fiber formation; cell adhesion; bone resorption; platelet activation; anatomical structure morphogenesis; mast cell chemotaxis; dendrite morphogenesis; positive regulation of Rho protein signal transduction; regulation of defense response to virus by virus; negative regulation of interleukin-23 production; T cell costimulation; cerebral cortex radially oriented cell migration; actin cytoskeleton organization and biogenesis; axon guidance; cell-matrix adhesion; localization within membrane; positive regulation of focal adhesion formation; actin filament polymerization; response to wounding; ephrin receptor signaling pathway; inflammatory response; regulation of hydrogen peroxide metabolic process; lamellipodium biogenesis; embryonic olfactory bulb interneuron precursor migration; intercellular junction assembly and maintenance; Wnt receptor signaling pathway, planar cell polarity pathway; positive regulation of phosphoinositide 3-kinase activity; engulfment of apoptotic cell; cell proliferation; G-protein coupled receptor protein signaling pathway; hyperosmotic response; positive regulation of actin filament polymerization; organization of an anatomical structure; auditory receptor cell morphogenesis; ruffle organization and biogenesis; innate immune response; negative regulation of receptor-mediated endocytosis; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; blood coagulation; cell motility; positive regulation of DNA replication