Human Prostate Specific Antigen (PSA) CLIA Kit | PSA clia kit

Human Prostate Specific Antigen (PSA) CLIA Kit

Cat. Num. AAA2708721

• Gene Names: KLK3; APS; PSA; hK3; KLK2A1
• Reactivity: Human
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Prostate Specific Antigen (PSA); Human Prostate Specific Antigen (PSA) CLIA Kit; Prostate Specific Antigen; KLK3; APS; KLK2A1; HK3; KLKB1; Seminin; Kallikrein-Related Peptidase 3; Semenogelase; g-Seminoprotein; P-30 Antigen; Plasma kallikrein; PSA clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Prostate Specific Antigen (PSA).; No significant cross-reactivity or interference between Prostate Specific Antigen (PSA) and analogues was observed.
• Sequence Length: 238

• Applicable Applications for PSA clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 54.9-40,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 19.1pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostate Specific Antigen (PSA) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostate Specific Antigen (PSA) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Prostate Specific Antigen (PSA). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Prostate Specific Antigen (PSA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Prostate Specific Antigen (PSA) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 71834853
• NCBI GeneID: 354
• NCBI Accession #: NP_001025218.1
• NCBI GenBank Nucleotide #: NP_001025218.1
• UniProt Accession #: P07288
• NCBI Official Full Name: prostate-specific antigen isoform 3 preproprotein
• NCBI Official Synonym Full Names: kallikrein related peptidase 3
• NCBI Official Symbol: KLK3
• NCBI Official Synonym Symbols: APS; PSA; hK3; KLK2A1
• NCBI Protein Information: prostate-specific antigen
• UniProt Protein Name: Prostate-specific antigen
• UniProt Gene Name: KLK3
• UniProt Synonym Gene Names: APS; PSA; Seminin
• UniProt Entry Name: KLK3_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. The gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. It encodes a single-chain glycoprotein, a protease which is synthesized in the epithelial cells of the prostate gland, and is present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. The serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Dec 2019]

Uniprot Description

PSA: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Forms heterodimer with SERPINA5. Inhibited by SERPINA5. Activity is strongly inhibited by Zn2+, 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins, which are avid zinc binders. Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein type: Secreted; Protease; EC 3.4.21.77; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: extracellular region; nucleus

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: negative regulation of angiogenesis; cellular protein metabolic process; proteolysis