Typical Testing Data/Standard Curve (for reference only)
Human Pappalysin 2 (PAPPA2) CLIA Kit | PAPPA2 clia kit
Human Pappalysin 2 (PAPPA2) CLIA Kit
Gene Names
PAPPA2; PAPPE; PLAC3; PAPP-E; PAPP-A2
Reactivity
Human
Synonyms
Pappalysin 2 (PAPPA2); Human Pappalysin 2 (PAPPA2) CLIA Kit; PAPP-A2; PAPPE; PLAC3; Placenta-Specific 3; Pregnancy-associated plasma protein A2; Pregnancy-associated plasma protein E1; PAPPA2 clia kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Pappalysin 2 (PAPPA2).
No significant cross-reactivity or interference between Pappalysin 2 (PAPPA2) and analogues was observed.
No significant cross-reactivity or interference between Pappalysin 2 (PAPPA2) and analogues was observed.
Sequence Length
827
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma and Other Biological Fluids
Detection Range
27.4-20,000pg/mL
Sensitivity
< 9.8pg/mL
Application
Chemiluminescent immunoassay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Pappalysin 2 (PAPPA2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Pappalysin 2 (PAPPA2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PAPPA2 clia kit
The microplate provided in this kit has been pre-coated with an antibody specific to Pappalysin 2 (PAPPA2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Pappalysin 2 (PAPPA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Pappalysin 2 (PAPPA2) level in the sample or standard.;
Product Categories/Family for PAPPA2 clia kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
92,136 Da
NCBI Official Full Name
Pappalysin 2
NCBI Official Synonym Full Names
pappalysin 2
NCBI Official Symbol
PAPPA2
NCBI Official Synonym Symbols
PAPPE; PLAC3; PAPP-E; PAPP-A2
NCBI Protein Information
pappalysin-2
UniProt Protein Name
Pappalysin-2
Protein Family
UniProt Gene Name
PAPPA2
UniProt Synonym Gene Names
PLAC3; PAPP-A2; PAPP-E
UniProt Entry Name
PAPP2_HUMAN
NCBI Description
This gene encodes a member of the pappalysin family of metzincin metalloproteinases. The encoded protein cleaves insulin-like growth factor-binding protein 5 and is thought to be a local regulator of insulin-like growth factor (IGF) bioavailability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]
Uniprot Description
PAPPA2: Metalloproteinase which specifically cleaves IGFBP-5. Shows limited proteolysis toward IGFBP-3. Belongs to the peptidase M43B family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.24.-; Protease; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1q25.2
Cellular Component: membrane; extracellular region; intracellular
Molecular Function: metallopeptidase activity; zinc ion binding
Biological Process: cellular protein metabolic process; regulation of cell growth; proteolysis; cell differentiation
Research Articles on PAPPA2
Similar Products
Product Notes
The Human PAPPA2 pappa2 (Catalog #AAA2001073) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2001073 CLIA Kit recognizes Human PAPPA2. It is sometimes possible for the material contained within the vial of "Pappalysin 2 (PAPPA2), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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