Human NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) CLIA Kit | NLRP3 clia kit

Human NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) CLIA Kit

Cat. Num. AAA2709211

• Reactivity: Human
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: NLR Family; Pyrin Domain Containing Protein 3 (NLRP3); Human NLR Family; Pyrin Domain Containing Protein 3 (NLRP3) CLIA Kit; Pyrin Domain Containing Protein 3; AVP; AGTAVPRL; AII; AII/AVP; C1orf7; CIAS1; CLR1.1; FCAS; FCU; MWS; NALP3; PYPAF1; Cryopyrin; Cold Induced Autoinflammatory Syndrome 1; Caterpiller-Like Receptor 1.1; NLRP3 clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NLR Family, Pyrin Domain Containing Protein 3 (NLRP3).; No significant cross-reactivity or interference between NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) and analogues was observed.
• Sequence Length: 920

• Applicable Applications for NLRP3 clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, cell lysates and other biological fluids
• Detection Range: 68.6-50,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 27.5pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to NLR Family, Pyrin Domain Containing Protein 3 (NLRP3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to NLR Family, Pyrin Domain Containing Protein 3 (NLRP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 1784638299
• NCBI Accession #: NP_899632
• NCBI GenBank Nucleotide #: NP_899632.2
• UniProt Accession #: Q96P20
• NCBI Official Full Name: NACHT, LRR and PYD domains-containing protein 3 isoform b
• UniProt Protein Name: NACHT, LRR and PYD domains-containing protein 3
• Protein Family: NACHT, LRR and PYD domains-containing protein
• UniProt Gene Name: NLRP3
• UniProt Synonym Gene Names: C1orf7; CIAS1; NALP3; PYPAF1; CLR1.1
• UniProt Entry Name: NALP3_HUMAN

Uniprot Description

NLRP3: May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1); also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS); also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA); also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation. Belongs to the NLRP family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 1q44

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; peptidoglycan binding; ATP binding

Biological Process: caspase activation; apoptosis; negative regulation of acute inflammatory response; positive regulation of caspase activity; interleukin-1 beta production; defense response; negative regulation of NF-kappaB import into nucleus; positive regulation of interleukin-1 beta secretion; signal transduction; protein oligomerization; activation of NF-kappaB transcription factor; negative regulation of interleukin-1 beta secretion; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; interleukin-18 production; innate immune response; interleukin-1 secretion; inflammatory response; detection of biotic stimulus; defense response to virus

Disease: Cinca Syndrome; Familial Cold Autoinflammatory Syndrome 1; Muckle-wells Syndrome