Western Blot (WB)
(PRIM2 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PRIM2 antibody detected the PRIM2 protein (arrow).)
Rabbit PRIM2 Polyclonal Antibody | anti-PRIM2 antibody
PRIM2 Antibody (Center)
Western Blot (WB)
(PRIM2 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PRIM2 antibody detected the PRIM2 protein (arrow).)
Western Blot (WB)
(PRIM2 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PRIM2 antibody detected the PRIM2 protein (arrow).)
by a complex chromosomal replication apparatus, in which DNA
polymerase alpha and primase are two key enzymatic components.
Primase, which is a heterodimer of a small subunit and a large
subunit, synthesizes small RNA primers for the Okazaki fragments
made during discontinuous DNA replication. The protein encoded by
this gene is the large, 58 kDa primase subunit. [provided by
RefSeq].
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Weiner, B.E., et al. J. Biol. Chem. 282(46):33444-33451(2007)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Mungall, A.J., et al. Nature 425(6960):805-811(2003)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the 58 kilodalton subunit of DNA primase, an enzyme that plays a key role in the replication of DNA. The encoded protein forms a heterodimer with a 49 kilodalton subunit. This heterodimer functions as a DNA-directed RNA polymerase to synthesize small RNA primers that are used to create Okazaki fragments on the lagging strand of the DNA. Alternative splicing of this gene results in multiple transcript variants. This gene has a related pseudogene, which is also present on chromosome 6. [provided by RefSeq, Apr 2014]
Uniprot Description
PRI2: synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The replication of DNA is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components.
Protein type: EC 2.7.7.-; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Transferase
Chromosomal Location of Human Ortholog: 6p12-p11.1
Cellular Component: nucleoplasm
Molecular Function: DNA primase activity; DNA binding; metal ion binding; 4 iron, 4 sulfur cluster binding
Biological Process: telomere maintenance via semi-conservative replication; DNA replication initiation; telomere maintenance via recombination; DNA replication, synthesis of RNA primer; DNA strand elongation during DNA replication; mitotic cell cycle; telomere maintenance; G1/S transition of mitotic cell cycle
Research Articles on PRIM2
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Product Notes
The PRIM2 prim2 (Catalog #AAA9206659) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 317-345. The PRIM2 Antibody (Center) reacts with Human (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's PRIM2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the PRIM2 prim2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRIM2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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