Western Blot (WB)
(Western blot analysis of NLK expression in A375 cell lysate.)
Rabbit anti-Human NLK Polyclonal Antibody | anti-NLK antibody
NLK Rabbit pAb
FC/FACS: 1:50
Western Blot (WB)
(Western blot analysis of NLK expression in A375 cell lysate.)
Western Blot (WB)
(Western blot analysis of NLK expression in A375 cell lysate.)
NCBI and Uniprot Product Information
Uniprot Description
NLK: Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK- SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Protein type: EC 2.7.11.24; Protein kinase, CMGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; nmo subfamily
Chromosomal Location of Human Ortholog: 17q11.2
Cellular Component: nucleoplasm; cytosol; nucleus
Molecular Function: MAP kinase activity; protein serine/threonine kinase activity; protein binding; ubiquitin protein ligase binding; magnesium ion binding; SH2 domain binding; transcription factor binding; ATP binding; protein kinase activity
Biological Process: negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; transcription, DNA-dependent; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; protein amino acid autophosphorylation; MAPKKK cascade; peptidyl-threonine phosphorylation; protein amino acid phosphorylation; serine phosphorylation of STAT3 protein
Research Articles on NLK
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Product Notes
The NLK nlk (Catalog #AAA4757584) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NLK Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NLK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 FC/FACS: 1:50. Researchers should empirically determine the suitability of the NLK nlk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NLK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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