Human D2R/DRD2 (Dopamine Receptor D2) CLIA Kit | D2R/DRD2 clia kit

Human D2R/DRD2 (Dopamine Receptor D2) CLIA Kit

Cat. Num. AAA2530496

• Gene Names: DRD2; D2R; D2DR
• Reactivity: Human
• Synonyms: D2R/DRD2 (Dopamine Receptor D2); Human D2R/DRD2 (Dopamine Receptor D2) CLIA Kit; D2R/DRD2 clia kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant D2R/DRD2. No significant cross-reactivity or interference between D2R/DRD2 and analogues was observed.
• Sequence Length: 443

• Assay Type: Sandwich
• Detection Range: 62.5-4000pg/mL
• Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for D2R/DRD2 clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of D2R/DRD2 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to D2R/DRD2. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for D2R/DRD2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain D2R/DRD2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of D2R/DRD2. You can calculate the concentration of D2R/DRD2 in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5921480
• NCBI GeneID: 1813
• UniProt Accession #: P14416; Q9NZR3; Q9UPA9
• Molecular Weight: 50,847 Da
• NCBI Official Full Name: dopamine receptor D2
• NCBI Official Synonym Full Names: dopamine receptor D2
• NCBI Official Symbol: DRD2
• NCBI Official Synonym Symbols: D2R; D2DR
• NCBI Protein Information: D(2) dopamine receptor
• UniProt Protein Name: D(2) dopamine receptor
• UniProt Gene Name: DRD2
• UniProt Entry Name: DRD2_HUMAN

NCBI Description

This gene encodes the D2 subtype of the dopamine receptor. This G-protein coupled receptor inhibits adenylyl cyclase activity. A missense mutation in this gene causes myoclonus dystonia; other mutations have been associated with schizophrenia. Alternative splicing of this gene results in two transcript variants encoding different isoforms. A third variant has been described, but it has not been determined whether this form is normal or due to aberrant splicing. [provided by RefSeq, Jul 2008]

Uniprot Description

DRD2: Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Defects in DRD2 are associated with dystonia type 11 (DYT11); also known as alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT11 is characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: synaptic vesicle membrane; nonmotile primary cilium; integral to plasma membrane; postsynaptic density; dendrite; dendritic spine; acrosome; perikaryon; cytosol; endocytic vesicle; axon; plasma membrane; nerve terminal; intracellular; lateral plasma membrane

Molecular Function: dopamine D2 receptor-like receptor activity; identical protein binding; ionotropic glutamate receptor binding; protein binding; protein homodimerization activity; potassium channel regulator activity; adrenoceptor activity; protein heterodimerization activity; dopamine binding; drug binding

Biological Process: response to nicotine; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of dopamine uptake; prepulse inhibition; response to toxin; positive regulation of receptor internalization; thermoregulation; regulation of phosphoprotein phosphatase activity; positive regulation of multicellular organism growth; adult walking behavior; dopamine metabolic process; dopamine receptor, adenylate cyclase inhibiting pathway; negative regulation of insulin secretion; protein localization; negative regulation of innate immune response; phosphatidylinositol metabolic process; negative regulation of blood pressure; response to drug; positive regulation of neuroblast proliferation; response to light stimulus; response to inactivity; cerebral cortex GABAergic interneuron migration; negative regulation of synaptic transmission, glutamatergic; regulation of sodium ion transport; arachidonic acid secretion; regulation of synaptic transmission, GABAergic; positive regulation of growth hormone secretion; G-protein coupled receptor internalization; reduction of cytosolic calcium ion concentration; activation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; regulation of long-term neuronal synaptic plasticity; synaptic transmission, dopaminergic; peristalsis; branching morphogenesis of a nerve; negative regulation of circadian sleep/wake cycle, sleep; response to morphine; locomotory behavior; behavioral response to ethanol; orbitofrontal cortex development; regulation of dopamine secretion; negative regulation of cell proliferation; synaptogenesis; behavioral response to cocaine; adenohypophysis development; visual learning; feeding behavior; nerve-nerve synaptic transmission; response to axon injury; circadian regulation of gene expression; negative regulation of dopamine secretion; response to iron ion; negative regulation of cell migration; associative learning; grooming behavior; positive regulation of cytokinesis; negative regulation of protein secretion; Wnt receptor signaling pathway; negative regulation of protein kinase B signaling cascade; striatum development; regulation of potassium ion transport; regulation of cAMP metabolic process; regulation of heart rate; sensory perception of smell; negative regulation of adenylate cyclase activity; response to amphetamine; auditory behavior; regulation of dopamine uptake; response to cocaine; cellular calcium ion homeostasis; regulation of systemic arterial blood pressure by neurological process; negative regulation of dopamine receptor signaling pathway; pigmentation; axonogenesis; long-term memory; positive regulation of G-protein coupled receptor protein signaling pathway; release of sequestered calcium ion into cytosol; response to hypoxia; regulation of synapse structural plasticity; dopamine receptor, phospholipase C activating pathway

Disease: Myoclonic Dystonia