Human Caspase 14 (CASP14) CLIA Kit | CASP14 clia kit

Human Caspase 14 (CASP14) CLIA Kit

Cat. Num. AAA2000532

• Reactivity: Human
• Synonyms: Caspase 14 (CASP14); Human Caspase 14 (CASP14) CLIA Kit; Caspase 14 Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 14; CASP14 clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Caspase 14 (CASP14).; No significant cross-reactivity or interference between Caspase 14 (CASP14) and analogues was observed.
• Sequence Length: 242

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 27.4-20,000pg/mL
• Sensitivity: < 10.56pg/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 14 (CASP14) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 14 (CASP14) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CASP14 clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Caspase 14 (CASP14). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Caspase 14 (CASP14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Caspase 14 (CASP14) level in the sample or standard.;

Product Categories/Family for CASP14 clia kit

Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 6912286
• NCBI GeneID: 23581
• NCBI Accession #: NP_036246.1
• NCBI GenBank Nucleotide #: NM_012114.2
• UniProt Accession #: P31944; O95823; Q3SYC9
• Molecular Weight: 27,680 Da
• NCBI Official Full Name: caspase-14
• NCBI Official Synonym Full Names: caspase 14, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP14
• NCBI Protein Information: caspase-14
• UniProt Protein Name: Caspase-14
• Protein Family: Caspase
• UniProt Gene Name: CASP14
• UniProt Synonym Gene Names: CASP-14
• UniProt Entry Name: CASPE_HUMAN

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This caspase has been shown to be processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. [provided by RefSeq, Jul 2008]

Uniprot Description

CASP14: Believed to be a non-apoptotic caspase which is involved in epidermal differentiation. Seems to play a role in keratinocyte differentiation and cornification. Probably regulates maturation of the epidermis by proteolytically processing filaggrin. Complex of unprocessed caspase-14 and processed 19 kDa (p19) and 10 kDa (p10) subunits. In undifferentiated keratinocytes under postconfluency growth conditions (in vitro). Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum). Expressed in keratinocytes of hair shaft and sebaceous glands. In psoriatic skin only expressed at very low levels. Belongs to the peptidase C14A family.

Protein type: EC 3.4.22.-; Protease

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: keratin filament; cytoplasm; nucleus

Molecular Function: cysteine-type endopeptidase activity

Biological Process: epidermis development; DNA damage response, signal transduction resulting in induction of apoptosis; keratinization; proteolysis