Human Apolipoprotein B (APOB) CLIA Kit | APOB clia kit

Human Apolipoprotein B (APOB) CLIA Kit

Cat. Num. AAA2025433

• Gene Names: APOB; FLDB; LDLCQ4
• Reactivity: Human
• Synonyms: Apolipoprotein B (APOB); Human Apolipoprotein B (APOB) CLIA Kit; Apo-B; FLDB; Apo B-100; Apo B-48; APOB clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Apolipoprotein B (APOB).; No significant cross-reactivity or interference between Apolipoprotein B (APOB) and analogues was observed.
• Sequence Length: 1670

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 6.86-5,000ng/mL
• Sensitivity: < 2.84ng/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein B (APOB) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein B (APOB) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOB clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein B (APOB). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Apolipoprotein B (APOB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Apolipoprotein B (APOB) level in the sample or standard.;

Product Categories/Family for APOB clia kit

Metabolic pathway; Endocrinology; Cardiovascular biology; Hepatology; Nutrition metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 553189
• NCBI GeneID: 338
• Molecular Weight: 515,605 Da
• NCBI Official Full Name: apolipoprotein B, partial
• NCBI Official Synonym Full Names: apolipoprotein B
• NCBI Official Symbol: APOB
• NCBI Official Synonym Symbols: FLDB; LDLCQ4
• NCBI Protein Information: apolipoprotein B-100
• UniProt Protein Name: Apolipoprotein B-100
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOB
• UniProt Synonym Gene Names: Apo B-100; Apo B-48
• UniProt Entry Name: APOB_HUMAN

NCBI Description

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]

Uniprot Description

APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.

Protein type: Carrier; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2p24-p23

Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum membrane; chylomicron; cell soma; intracellular membrane-bound organelle; endoplasmic reticulum lumen; cytoplasm; early endosome; plasma membrane; extracellular region; endosome membrane; cytosol; actin cytoskeleton

Molecular Function: heparin binding; protein binding; low-density lipoprotein receptor binding; cholesterol transporter activity; phospholipid binding

Biological Process: lipoprotein catabolic process; phototransduction, visible light; response to lipopolysaccharide; response to carbohydrate stimulus; post-embryonic development; response to selenium ion; triacylglycerol catabolic process; retinoid metabolic process; transmembrane transport; nervous system development; receptor-mediated endocytosis; cholesterol metabolic process; cholesterol transport; in utero embryonic development; response to virus; regulation of cholesterol biosynthetic process; cholesterol efflux; lipoprotein metabolic process; sperm motility; cholesterol homeostasis; fertilization; lipoprotein transport; lipoprotein biosynthetic process; artery morphogenesis; spermatogenesis; triacylglycerol mobilization; blood coagulation; leukocyte migration

Disease: Hypercholesterolemia, Autosomal Dominant, Type B; Hypobetalipoproteinemia, Familial, 1