Human Apolipoprotein A4 (APOA4) CLIA Kit | APOA4 clia kit

Human Apolipoprotein A4 (APOA4) CLIA Kit

Cat. Num. AAA2000785

• Reactivity: Human
• Synonyms: Apolipoprotein A4 (APOA4); Human Apolipoprotein A4 (APOA4) CLIA Kit; ApoA-IV; ApoAIV; Apo-A4; APOA4 clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Apolipoprotein A4 (APOA4).; No significant cross-reactivity or interference between Apolipoprotein A4 (APOA4) and analogues was observed.
• Sequence Length: 396

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.21-150ng/mL
• Sensitivity: < 0.08ng/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein A4 (APOA4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein A4 (APOA4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOA4 clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A4 (APOA4). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Apolipoprotein A4 (APOA4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Apolipoprotein A4 (APOA4) level in the sample or standard.;

Product Categories/Family for APOA4 clia kit

Metabolic pathway; Endocrinology; Cardiovascular biology; Hepatology; Gastroenterology; Nutrition metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 71773110
• NCBI GeneID: 337
• NCBI Accession #: NP_000473.2
• NCBI GenBank Nucleotide #: NM_000482.3
• UniProt Accession #: P06727; Q14CW8; Q6Q787; A8MSL6
• Molecular Weight: 45,399 Da
• NCBI Official Full Name: apolipoprotein A-IV
• NCBI Official Synonym Full Names: apolipoprotein A-IV
• NCBI Official Symbol: APOA4
• NCBI Protein Information: apolipoprotein A-IV
• UniProt Protein Name: Apolipoprotein A-IV
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA4
• UniProt Synonym Gene Names: Apo-AIV; ApoA-IV
• UniProt Entry Name: APOA4_HUMAN

NCBI Description

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq, Jul 2008]

Uniprot Description

APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Secreted; Lipid-binding; Secreted, signal peptide; Cell adhesion; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: extracellular space; chylomicron; cell surface; endoplasmic reticulum lumen; early endosome; extracellular region; cytosol

Molecular Function: antioxidant activity; lipid transporter activity; protein binding; copper ion binding; protein homodimerization activity; cholesterol transporter activity; cholesterol binding; phosphatidylcholine binding; lipid binding

Biological Process: multicellular organismal lipid catabolic process; cholesterol metabolic process; phototransduction, visible light; positive regulation of fatty acid biosynthetic process; removal of superoxide radicals; protein-lipid complex assembly; neurite regeneration; lipid homeostasis; lipoprotein metabolic process; cholesterol efflux; lipid transport; cholesterol biosynthetic process; phosphatidylcholine metabolic process; leukocyte adhesion; cholesterol homeostasis; reverse cholesterol transport; innate immune response in mucosa; phospholipid efflux; regulation of cholesterol absorption; response to lipid hydroperoxide; hydrogen peroxide catabolic process; positive regulation of lipoprotein lipase activity; retinoid metabolic process; regulation of cholesterol transport