Vascular Endothelial Growth Factor Receptor 1 Active Protein | FLT1 active protein

Recombinant Human Vascular Endothelial Growth Factor Receptor 1 Protein

Cat. Num. AAA2556738

• Gene Names: FLT1; FLT; FLT-1; VEGFR1; VEGFR-1
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Vascular Endothelial Growth Factor Receptor 1; Recombinant Human Vascular Endothelial Growth Factor Receptor 1 Protein; Vascular endothelial growth factor receptor 1; VEGFR-1; Fms-like tyrosine kinase 1; FLT-1; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; Vascular permeability factor receptor; FLT1 active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH 7.4.
• Sequence Length: 1338

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fragment: Ser27-Asn756
• Fusion Tag: C-Fc
• Activity: Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC). (Conn, G.?et?al.?,1990, Proc. Natl. Acad. Sci. USA 87:1323.)The ED50?for this effect is typically 10-40 ng/mL in the presence of 10 ng/mL human VEGF165.
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days.; Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for FLT1 active protein

Protein Construction: Recombinant Human Vascular Endothelial Growth Factor Receptor 1 is produced by our Mammalian expression system and the target gene encoding Ser27-Asn756 is expressed with a Fc tag at the C-terminus.

Background: Human Vascular endothelial growth factor receptor 1(VEGFR-1, FLT-1) is a member of the the class III subfamily of receptor tyrosine kinases (RTKs) and Tyr protein kinase family and CSF-1/PDGF receptor subfamily. VEGFR-1 is widely expressed in human tissues including normal lung, placenta, liver, kidney, heart and brain tissues. It is specifically expressed in most of the vascular endothelial cellsand peripheral blood monocytes. VEGFR-1 contains seven Ig-like C2-type domains and one protein kinase domain. VEGFR-1is an essential receptor tyrosine kinase and plays an important role in theregulation of VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. It is also mediators of neurotrophic activity and regulators of hematopoietic development. VEGFR-1 is a receptor for VEGF, VEGFB and PGF. It has a tyrosine-protein kinase activity. Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF.It may play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells and promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. VEGFR-1 can also promote PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro).

NCBI and Uniprot Product Information

• NCBI GI #: 156104876
• NCBI GeneID: 2321
• NCBI Accession #: NP_002010.2
• NCBI GenBank Nucleotide #: NM_002019.4
• UniProt Accession #: P17948; O60722; A3E342; A3E344; A8KA71; B0LPF1; B2BF46; B2BF47; B2BF48; B3FR89; B5A923; F5H5L6
• Molecular Weight: Molecular Mass: 109.0kDa; Actual Protein Molecular Mass: 150-160kDa
• NCBI Official Full Name: vascular endothelial growth factor receptor 1 isoform 1
• NCBI Official Synonym Full Names: fms related tyrosine kinase 1
• NCBI Official Symbol: FLT1
• NCBI Official Synonym Symbols: FLT; FLT-1; VEGFR1; VEGFR-1
• NCBI Protein Information: vascular endothelial growth factor receptor 1
• UniProt Protein Name: Vascular endothelial growth factor receptor 1
• Protein Family: Vascular endothelial growth factor receptor
• UniProt Gene Name: FLT1
• UniProt Synonym Gene Names: FLT; FRT; VEGFR1; VEGFR-1; FLT-1; FLT

NCBI Description

This gene encodes a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig)-like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.[provided by RefSeq, May 2009]

Uniprot Description

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro). Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Likewise, isoforms lacking a transmembrane domain, such as isoform 2, isoform 3 and isoform 4, may function as decoy receptors for VEGFA. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC and YES1, and may also phosphorylate CBL. Isoform 1 phosphorylates PLCG. Promotes phosphorylation of AKT1 at 'Ser-473'. Promotes phosphorylation of PTK2/FAK1. Isoform 7 has a truncated kinase domain; it increases phosphorylation of SRC at 'Tyr-418' by unknown means and promotes tumor cell invasion.