Transferrin Active Protein | TF active protein

Recombinant Human Transferrin Protein

Cat. Num. AAA2556684

• Gene Names: TF; TFQTL1; PRO1557; PRO2086; HEL-S-71p
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Transferrin; Recombinant Human Transferrin Protein; Serotransferrin; Beta-1 metal-binding globulin; Siderophilin; TF; TF active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH 7.4.
• Sequence Length: 698

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fragment: Val20-Pro698
• Fusion Tag: C-6His
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD71 at 10 mug/ml (100 mul/well) can bind human Transferrin. The EC50 of human Transferrin is 5.6 ng/mL. 2. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is typically 0.01-0.04 mug/mL.
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.

SDS-Page

Related Product Information for TF active protein

Protein Construction: Recombinant Human Transferrin is produced by our Mammalian expression system and the target gene encoding Val20-Pro698 is expressed with a 6His tag at the C-terminus. 

Background: Serotransferrin belongs to transferrin family, and contains 2 transferrin-like domains. The protein is a secreted protein, and expressed by the liver and secreted in plasma. Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.

NCBI and Uniprot Product Information

• NCBI GI #: 4557871
• NCBI GeneID: 7018
• NCBI Accession #: NP_001054.1
• NCBI GenBank Nucleotide #: NM_001063.3
• UniProt Accession #: P02787; O43890; Q1HBA5; Q9NQB8; Q9UHV0
• Molecular Weight: Molecular Mass: 76.2kDa; Actual Protein Molecular Mass: 85kDa
• NCBI Official Full Name: serotransferrin isoform 1
• NCBI Official Synonym Full Names: transferrin
• NCBI Official Symbol: TF
• NCBI Official Synonym Symbols: TFQTL1; PRO1557; PRO2086; HEL-S-71p
• NCBI Protein Information: serotransferrin
• UniProt Protein Name: Serotransferrin
• Protein Family: Transferrin
• UniProt Gene Name: TF
• UniProt Synonym Gene Names: Transferrin

NCBI Description

This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009]

Uniprot Description

Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.