HLA Class II Histocompatibility Antigen gamma Active Protein | CD74 active protein

Recombinant Mouse HLA Class II Histocompatibility Antigen gamma Chain Protein

Cat. Num. AAA2556681

• Gene Names: CD74; II; p33; DHLAG; HLADG; Ia-GAMMA
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: HLA Class II Histocompatibility Antigen gamma; Recombinant Mouse HLA Class II Histocompatibility Antigen gamma Chain Protein; Cluster of Differentiation 74; CD74 antigen; CD74 molecule; major histocompatibility complex; class II invariant chain; DHLAG gamma chain of class II antigens; HLA class II histocompatibility antigen gamma chain; HLADG; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ia-GAMMA; MHC HLA-DR gamma chain; CD74; DHLAG; Ia-gamma; INVG34; CD74 active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH 7.4.
• Sequence Length: 232

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fragment: Gln56-Leu215
• Fusion Tag: C-6His
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD74 at 5 mug/ml (100 mul/well) can bind biotinylated human CTSL1 with a linear range of 3.2-400 ng/ml.
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days.; Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

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Related Product Information for CD74 active protein

Protein Construction: Recombinant Mouse HLA class II histocompatibility antigen gamma chain is produced by our Mammalian expression system and the target gene encoding Gln56-Leu215 is expressed with a 6His tag at the C-terminus.

Background: Mouse HLA class II histocompatibility antigen gamma chain (CD74), is a single-pass type II membrane glycoprotein which contains 1 thyroglobulin type-1 domain. Mouse CD74 shares 75% and 88% aa sequence identity with human and rat CD74, respectively. CD74 plays an important role in adaptive immunity, inflammation, and cancer. It plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to compartments where peptide loading of class II takes place.CD74 also associates with CD44 and binds with high affinity to the cytokine MIF, leading to inflammatory leukocyte responses, protection from tissue fibrosis, B cell proliferative and survival signaling, and the up-regulation of angiogenic factors in endometrial stromal cells.

NCBI and Uniprot Product Information

• NCBI GI #: 10835071
• NCBI GeneID: 972
• NCBI Accession #: NP_004346.1
• NCBI GenBank Nucleotide #: NM_004355.3
• UniProt Accession #: P04233; Q14597; Q29832; Q5U0J8; Q8SNA0; Q8WLP6; A8K7R1; B4DNE8; D3DQG3; D3DQG4
• Molecular Weight: Molecular Mass: 19.4kDa; Actual Protein Molecular Mass: 25-30kDa
• NCBI Official Full Name: HLA class II histocompatibility antigen gamma chain isoform b
• NCBI Official Synonym Full Names: CD74 molecule
• NCBI Official Symbol: CD74
• NCBI Official Synonym Symbols: II; p33; DHLAG; HLADG; Ia-GAMMA
• NCBI Protein Information: HLA class II histocompatibility antigen gamma chain
• UniProt Protein Name: HLA class II histocompatibility antigen gamma chain
• Protein Family: H-2 class II histocompatibility antigen
• UniProt Gene Name: CD74
• UniProt Synonym Gene Names: DHLAG; Ii

NCBI Description

The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.