Testing Data
(Staining of cells with CD31 in red and vWF in green. Cells were counterstained with DAPI (cell nuclei). CD31 is distributed on cell membrane whereas vWF is in cytoplasm as expected.)
Large Intestine Microvascular Endothelial Cells Cell Line
Human Large Intestine Microvascular Endothelial Cells
Reactivity
Human
Applications
Cell Assay
Synonyms
Large Intestine Microvascular Endothelial Cells; Human Large Intestine Microvascular Endothelial Cells; Large Intestine Microvascular Endothelial Cells cell line
Reactivity
Human
Applicable Applications for Large Intestine Microvascular Endothelial Cells cell line
Cell Assay
Preparation and Storage
Keep frozen in liquid nitrogen (Dry Ice) until plating
Testing Data
(Staining of cells with CD31 in red and vWF in green. Cells were counterstained with DAPI (cell nuclei). CD31 is distributed on cell membrane whereas vWF is in cytoplasm as expected.)
Testing Data
(Staining of cells with CD31 in red and vWF in green. Cells were counterstained with DAPI (cell nuclei). CD31 is distributed on cell membrane whereas vWF is in cytoplasm as expected.)
Testing Data
(Human Large Intestine Microvascular Endothelial Cells in two days in culture.)
Testing Data
(Human Large Intestine Microvascular Endothelial Cells in two days in culture.)
Related Product Information for Large Intestine Microvascular Endothelial Cells cell line
HLIMECs were initiated by elutriation from dissociated normal large intestine tissue. Passage 3 cells are shipped cryopreserved. ENDO-Growth Medium (MED001) is recommended for culture. Cells have an average additional population doubling levels >16 when cultured.
Characterization of the cells
Cytoplasmic VWF/Factor VIII: >95% positive by immunofluorescence
Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
Cytoplasmic PECAM1 >95% positive by immunofluorescence
HLIMECs are negative for HIV-1, HBV, HCV, and mycoplasma.
Characterization of the cells
Cytoplasmic VWF/Factor VIII: >95% positive by immunofluorescence
Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
Cytoplasmic PECAM1 >95% positive by immunofluorescence
HLIMECs are negative for HIV-1, HBV, HCV, and mycoplasma.
Product Categories/Family for Large Intestine Microvascular Endothelial Cells cell line
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Product Notes
The Large Intestine Microvascular Endothelial Cells (Catalog #AAA556020) is a Cell Line and is intended for research purposes only. The product is available for immediate purchase. The Human Large Intestine Microvascular Endothelial Cells reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Large Intestine Microvascular Endothelial Cells can be used in a range of immunoassay formats including, but not limited to, Cell Assay. Researchers should empirically determine the suitability of the Large Intestine Microvascular Endothelial Cells for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Large Intestine Microvascular Endothelial Cells, Cell Line" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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