Western Blot (WB)
(Western blot analysis of extracts from K562 cells, treated with UV (30mins), using PBK/TOPK (Phospho-Thr9) antibody.)
anti-Human PBK/TOPK Antibody | anti-PBK/TOPK antibody
PBK/TOPK (Phospho-Thr9) Antibody
Gene Names
PBK; SPK; CT84; TOPK; HEL164; Nori-3
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
PBK/TOPK; PBK/TOPK (Phospho-Thr9) Antibody; EC 2.7.12.2; MPAKK-like protein kinase cap site form 2; NORI-3; TOPK; anti-PBK/TOPK antibody
Reactivity
Human
Specificity
PBK/TOPK (Phospho-Thr9) antibody detects endogenous levels of PBK/TOPK only when phosphorylated at threonine 9.
Purity/Purification
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
333
Applicable Applications for anti-PBK/TOPK antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:40000
ELISA: 1:40000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human PBK/TOPK around the phosphorylation site of threonine 9 (F-K-TP-P-S).
All Sites
Human: Thr9
Preparation and Storage
Store at-20 degree C for 1 year.
Western Blot (WB)
(Western blot analysis of extracts from K562 cells, treated with UV (30mins), using PBK/TOPK (Phospho-Thr9) antibody.)
Western Blot (WB)
(Western blot analysis of extracts from K562 cells, treated with UV (30mins), using PBK/TOPK (Phospho-Thr9) antibody.)
Product Categories/Family for anti-PBK/TOPK antibody
References
Abe Y., J. Biol. Chem. 275:21525-21531(2000). Gaudet S., Proc. Natl. Acad. Sci. U.S.A. 97:5167-5172(2000). Zhao S., Int. J. Biochem. Cell Biol. 33:631-636(2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,211 Da
NCBI Official Full Name
lymphokine-activated killer T-cell-originated protein kinase isoform 2
NCBI Official Synonym Full Names
PDZ binding kinase
NCBI Official Symbol
PBK
NCBI Official Synonym Symbols
SPK; CT84; TOPK; HEL164; Nori-3
NCBI Protein Information
lymphokine-activated killer T-cell-originated protein kinase
UniProt Protein Name
Lymphokine-activated killer T-cell-originated protein kinase
UniProt Gene Name
PBK
UniProt Synonym Gene Names
TOPK; CT84; SPK
NCBI Description
This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Uniprot Description
Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
Research Articles on PBK/TOPK
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Product Notes
The PBK/TOPK pbk (Catalog #AAA859610) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The PBK/TOPK (Phospho-Thr9) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PBK/TOPK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:40000. Researchers should empirically determine the suitability of the PBK/TOPK pbk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PBK/TOPK, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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