anti-Human MYT1 Antibody | anti-MYT1 antibody

MYT1 (Phospho-Ser83) Antibody

Cat. Num. AAA854364

• Gene Names: PKMYT1; MYT1; PPP1R126
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: MYT1; MYT1 (Phospho-Ser83) Antibody; EC 2.7.11.1; kinase Myt1; Kinase MYT1; Membrane-associated tyrosine-and threonine-specific cdc2-inhibitory kinase; MYT1 kinase; Myt1 protein; PKMYT1; PMYT1; anti-MYT1 antibody

• Reactivity: Human
• Specificity: MYT1 (Phospho-Ser83) antibody detects endogenous levels of MYT1 only when phosphorylated at serine 83.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 430

• Applicable Applications for anti-MYT1 antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:5000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human MYT1 around the phosphorylation site of serine 83 (R-V-SP-F-R).
• All Sites: Human: Ser83
• Preparation and Storage: Store at-20 degree C for 1 year.

Testing Data

(P-peptide-+)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MYT1 (Phospho-Ser83) antibody. MYT1 (Phospho-Ser83) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

Product Categories/Family for anti-MYT1 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Booher R.N., J. Biol. Chem. 272:22300-22306(1997). Liu F., Mol. Cell. Biol. 17:571-583(1997). Nousiainen M., Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 386642875
• NCBI GeneID: 9088
• NCBI Accession #: NP_001245379.1
• NCBI GenBank Nucleotide #: NM_001258450.1
• UniProt Accession #: Q99640; O14731; Q7LE24; Q8TCM9; B3KUN8; B4DXD4; D3DUA4; F8W164; I3L1V2
• Molecular Weight: 47,284 Da
• NCBI Official Full Name: membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase isoform 3
• NCBI Official Synonym Full Names: protein kinase, membrane associated tyrosine/threonine 1
• NCBI Official Symbol: PKMYT1
• NCBI Official Synonym Symbols: MYT1; PPP1R126
• NCBI Protein Information: membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
• UniProt Protein Name: Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
• Protein Family: Myelin transcription factor
• UniProt Gene Name: PKMYT1
• UniProt Synonym Gene Names: MYT1

NCBI Description

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.