Immunohistochemistry (IHC)
((0.03ug/ml) staining of HeLa (A), HepG2 (B) and K562 (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
anti-Human MDH2 Antibody | anti-MDH2 antibody
Goat anti-MDH2 (aa221-232) Antibody
Gene Names
MDH2; MDH; MOR1; M-MDH; EIEE51; MGC:3559
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
MDH2; Goat anti-MDH2 (aa221-232) Antibody; malate dehydrogenase 2; NAD (mitochondrial); M-MDH; MDH; MGC:3559; MOR1; malate dehydrogenase; mitochondrial; anti-MDH2 antibody
Reactivity
Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
338
Applicable Applications for anti-MDH2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Pep-ELISA: antibody detection limit dilution 1:16000.
WB: Approx 36-37kDa band observed in lysates of cell lines HeLa, HepG2 and K562, and approx 35kDa band observed in Human Heart and Liver lysates. Recommended concentration: 0.01-0.03ug/ml.
WB: Approx 36-37kDa band observed in lysates of cell lines HeLa, HepG2 and K562, and approx 35kDa band observed in Human Heart and Liver lysates. Recommended concentration: 0.01-0.03ug/ml.
Immunogen Sequence
Peptide with sequence C-DQLTALTGRIQE, from the internal region of the protein sequence according to NP_005909.2.
Peptide Sequence + C
C-DQLTALTGRIQE
Epitope
Internal Region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Immunohistochemistry (IHC)
((0.03ug/ml) staining of HeLa (A), HepG2 (B) and K562 (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((0.03ug/ml) staining of HeLa (A), HepG2 (B) and K562 (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((0.03ug/ml) staining of Human Heart (A) and Liver (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((0.03ug/ml) staining of Human Heart (A) and Liver (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35.5kDa
NCBI Official Full Name
malate dehydrogenase, mitochondrial isoform 1
NCBI Official Synonym Full Names
malate dehydrogenase 2
NCBI Official Symbol
MDH2
NCBI Official Synonym Symbols
MDH; MOR1; M-MDH; EIEE51; MGC:3559
NCBI Protein Information
malate dehydrogenase, mitochondrial
UniProt Protein Name
Malate dehydrogenase, mitochondrial
Protein Family
UniProt Gene Name
MDH2
NCBI Description
Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]
Research Articles on MDH2
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Product Notes
The MDH2 mdh2 (Catalog #AAA423817) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MDH2 (aa221-232) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MDH2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Pep-ELISA: antibody detection limit dilution 1:16000. WB: Approx 36-37kDa band observed in lysates of cell lines HeLa, HepG2 and K562, and approx 35kDa band observed in Human Heart and Liver lysates. Recommended concentration: 0.01-0.03ug/ml. Researchers should empirically determine the suitability of the MDH2 mdh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MDH2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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