anti-Human IL2 Antibody | anti-IL2 antibody

IL2 Antibody

Cat. Num. AAA8516012

• Gene Names: IL2; IL-2; TCGF; lymphokine
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Immunogen affinity purified
• Synonyms: IL2; IL2 Antibody; IL-2; TCGF; lymphokine; anti-IL2 antibody

• Reactivity: Human
• Specificity: IL2 Antibody detects endogenous levels of total IL2
• Purity/Purification: Immunogen affinity purified
• Form/Format: In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 153

• Applicable Applications for anti-IL2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; ICC 1:50-1:200
• Immunogen: Recombinant protein of human IL2
• Preparation and Storage: Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis of CTLL-2 cell lysate using IL-2 antibody.)

Related Product Information for anti-IL2 antibody

IL-2 is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1, 2). IL-2 also promotes peripheral development of Treg cells (4, 5). Conversely, IL-2 is involved in activation induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells (3). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Ralpha, IL-2Rbeta and the common gamma chain, gamma c (1, 2). IL-2 Ralpha binds exclusively to IL-2 with low affinity and increases binding affinity of the whole receptor complex including IL-2Rbeta and gamma c subunits. IL-2Rbeta is also used by IL-15 (1, 2). The common gamma c is used by other cytokines including IL-4, IL-7, IL-9, IL-15, and IL-21 (1, 2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5 and the PI3K/Akt pathways (1, 2).

NCBI and Uniprot Product Information

• NCBI GI #: 28178861
• NCBI GeneID: 3558
• NCBI Accession #: NP_000577.2
• NCBI GenBank Nucleotide #: NM_000586.3
• UniProt Accession #: P60568; P01585
• Molecular Weight: 18kDa
• NCBI Official Full Name: interleukin-2
• NCBI Official Synonym Full Names: interleukin 2
• NCBI Official Symbol: IL2
• NCBI Official Synonym Symbols: IL-2; TCGF; lymphokine
• NCBI Protein Information: interleukin-2
• UniProt Protein Name: Interleukin-2
• Protein Family: Interleukin
• UniProt Gene Name: IL2
• UniProt Synonym Gene Names: IL-2; TCGF

NCBI Description

The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]

Uniprot Description

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.