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Immunohistochemistry (IHC) (Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using DP-1 antibody.)

anti-Human DP-1 Antibody | anti-DP-1 antibody

DP-1 Antibody

Gene Names
TFDP1; DP1; DILC; Dp-1; DRTF1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
DP-1; DP-1 Antibody; E2F DIMERIZATION PARTNER 1; TRANSCRIPTION FACTOR DP1; TRANSCRIPTION FACTOR; SEQUENCE-SPECIFIC; DRTF1; transcription factor Dp-1; anti-DP-1 antibody
Ordering
For Research Use Only!
Reactivity
Human
Specificity
DP-1 antibody detects endogenous levels of total DP-1 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
410
Applicable Applications for anti-DP-1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:5000
Immunogen
The antiserum was produced against synthesized peptide derived from human DP-1.
Preparation and Storage
Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using DP-1 antibody.)

Immunohistochemistry (IHC) (Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using DP-1 antibody.)

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, using DP-1 antibody.)

Western Blot (WB) (Western blot analysis of extracts from HeLa cells, using DP-1 antibody.)

Immunofluorescence (IF)

(Forskolin +- Immunofluorescence analysis of HeLa cells, treated with Forskolin (40nM, 30mins), using DP-1 antibody.)

Immunofluorescence (IF) (Forskolin +- Immunofluorescence analysis of HeLa cells, treated with Forskolin (40nM, 30mins), using DP-1 antibody.)
References
K Helin, Genes & Dev., Oct 1993; 7: 1850-1861. B Shan, Cell Growth Differ., Jun 1996; 7: 689. Hironori Ishida, J. Biol. Chem., Jul 2005; 280: 24642-24648. Wing Y. Chang, J. Biol. Chem., Dec 2004; 279: 51343-51353.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34,921 Da
NCBI Official Full Name
transcription factor Dp-1
NCBI Official Synonym Full Names
transcription factor Dp-1
NCBI Official Symbol
TFDP1
NCBI Official Synonym Symbols
DP1; DILC; Dp-1; DRTF1
NCBI Protein Information
transcription factor Dp-1
UniProt Protein Name
Transcription factor Dp-1
UniProt Gene Name
TFDP1
UniProt Synonym Gene Names
DP1; DRTF1

NCBI Description

This gene encodes a member of a family of transcription factors that heterodimerize with E2F proteins to enhance their DNA-binding activity and promote transcription from E2F target genes. The encoded protein functions as part of this complex to control the transcriptional activity of numerous genes involved in cell cycle progression from G1 to S phase. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1, 15, and X.[provided by RefSeq, Jan 2009]

Uniprot Description

Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication (PubMed:8405995, PubMed:7739537). The E2F1:DP complex appears to mediate both cell proliferation and apoptosis. Blocks adipocyte differentiation by repressing CEBPA binding to its target gene promoters (PubMed:20176812).

Research Articles on DP-1

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Product Notes

The DP-1 tfdp1 (Catalog #AAA852532) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The DP-1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DP-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000. Researchers should empirically determine the suitability of the DP-1 tfdp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DP-1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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