anti-Human CD3EAP Antibody | anti-CD3EAP antibody

CD3EAP Antibody

Cat. Num. AAA8514709

• Gene Names: CD3EAP; ASE1; CAST; ASE-1; PAF49
• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen affinity purified
• Synonyms: CD3EAP; CD3EAP Antibody; antisense to ERCC-1; CAST; CD3-epsilon-associated protein; CD3e-associated protein; DNA-directed RNA polymerase I RPA34; RNA polymerase I-associated factor PAF49; RPA34; anti-CD3EAP antibody

• Reactivity: Human
• Specificity: CD3EAP Antibody detects endogenous levels of total CD3EAP
• Purity/Purification: Immunogen affinity purified
• Form/Format: In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 512

• Applicable Applications for anti-CD3EAP antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthesized peptide
• Preparation and Storage: Store at-20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from A549 cells, using CD3EAP antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 662236821
• NCBI GeneID: 10849
• NCBI Accession #: NP_001284519.1
• NCBI GenBank Nucleotide #: NM_001297590.1
• UniProt Accession #: O15446; Q32N11; Q7Z5U2; Q9UPF6
• Molecular Weight: 55 KD
• NCBI Official Full Name: DNA-directed RNA polymerase I subunit RPA34 isoform 1
• NCBI Official Synonym Full Names: CD3e molecule associated protein
• NCBI Official Symbol: CD3EAP
• NCBI Official Synonym Symbols: ASE1; CAST; ASE-1; PAF49
• NCBI Protein Information: DNA-directed RNA polymerase I subunit RPA34
• UniProt Protein Name: DNA-directed RNA polymerase I subunit RPA34
• UniProt Gene Name: CD3EAP
• UniProt Synonym Gene Names: ASE1; CAST; PAF49; ASE-1; CAST; CD3E-associated protein

Uniprot Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.MiscellaneousIt is in an antisense orientation to and overlaps the gene of the DNA repair enzyme ERCC1. This gene overlap is conserved in mouse, suggesting an important biological function.CautionIt is not known whether the so-called human ASE1 and human CAST proteins represent two sides of a single gene product with sharply different functional characteristics. Experiments done with the mouse homolog protein are in favor of an implication of this gene in rRNA transcription instead of T-cell receptor signaling.