Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, treated with PMA (125ng/ml, 30mins), using CD32 (Ab-292) antibody.)
anti-Human CD32 Antibody | anti-CD32 antibody
CD32 (Ab-292) Antibody
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
CD32; CD32 (Ab-292) Antibody; CDW32; FC gamma receptor IIB; FC-gamma RII; FC-gamma RII-B; FC-gamma-RIIB; FCG2; FCG2B; FCGR2; FCGR2B; FCRII; FCgammaRIIB; IGFR2; IGG FC receptor II beta; IGG FC receptor II-B; LY-17; Low affinity immunoglobulin gamma FC region receptor II precursor; Low affinity immunoglobulin gamma FC region receptor II-B precursor; anti-CD32 antibody
Reactivity
Human
Specificity
CD32 (Ab-292) antibody detects endogenous levels of total CD32 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
290
Applicable Applications for anti-CD32 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:1000
ELISA: 1:1000
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human CD32 around the phosphorylation site of tyrosine 292 (I-T-YP-S-L).
Preparation and Storage
Store at-20 degree C for 1 year.
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, treated with PMA (125ng/ml, 30mins), using CD32 (Ab-292) antibody.)
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, treated with PMA (125ng/ml, 30mins), using CD32 (Ab-292) antibody.)
Product Categories/Family for anti-CD32 antibody
References
Belen de Andrés. Blood, Aug 1997; 90: 1267-1274. M Groger. J. Immunol., Feb 1996; 156: 1549. Diana Metes. Blood, Apr 1998; 91: 2369-2380. Belen de Andres. Blood, Oct 1998; 92: 2823-2829.
NCBI and Uniprot Product Information
Molecular Weight
31,873 Da
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor II-b
Protein Family
UniProt Gene Name
FCGR2B
UniProt Synonym Gene Names
CD32; FCG2; IGFR2; IgG Fc receptor II-b; Fc-gamma-RIIb; FcRII-b
Uniprot Description
Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.CautionHas sometimes been attributed to correspond to FcR-IIC.
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Product Notes
The CD32 fcgr2b (Catalog #AAA8500366) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The CD32 (Ab-292) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD32 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:1000. Researchers should empirically determine the suitability of the CD32 fcgr2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD32, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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