anti-Human BRCA1 Antibody | anti-BRCA1 antibody

BRCA1 (Phospho-Ser1423) Antibody

Cat. Num. AAA858950

• Gene Names: BRCA1; IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: BRCA1; BRCA1 (Phospho-Ser1423) Antibody; Breast cancer type 1 susceptibility protein; anti-BRCA1 antibody

• Reactivity: Human
• Specificity: BRCA1 (phospho-Ser1423) antibody detects endogenous levels of BRCA1 only when phosphorylated at serine 1423.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 1863

• Applicable Applications for anti-BRCA1 antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:4000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human BRCA1 around the phosphorylation site of serine 1423 (H-G-SP-Q-P).
• All Sites: Human: Ser1423
• Preparation and Storage: Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BRCA1 (phospho-Ser1423) antibody.)

Product Categories/Family for anti-BRCA1 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Kim ST, et al. (2002) Genes Dev; 16(5): 560-570. Arlt MF, et al. (2004) Mol Cell Biol; 24(15): 6701-6709. Zhang J, et al. (2004) Mol Cell Biol; 24(2): 708-718. Greenberg RA, et al. (2006) Genes Dev; 20(1): 34-46. Tibbetts RS, et al. (2000) Genes Dev; 14(23): 2989-3002.

NCBI and Uniprot Product Information

• NCBI GI #: 6552299
• NCBI GeneID: 672
• NCBI Accession #: NP_009225.1
• NCBI GenBank Nucleotide #: NM_007294.3
• UniProt Accession #: P38398; O15129; Q1RMC1; Q3LRJ0; Q3LRJ6; Q6IN79; Q7KYU9; E9PFZ0
• Molecular Weight: 202,342 Da
• NCBI Official Full Name: breast cancer type 1 susceptibility protein isoform 1
• NCBI Official Synonym Full Names: BRCA1, DNA repair associated
• NCBI Official Symbol: BRCA1
• NCBI Official Synonym Symbols: IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53
• NCBI Protein Information: breast cancer type 1 susceptibility protein
• UniProt Protein Name: Breast cancer type 1 susceptibility protein
• Protein Family: BRCA1-A complex
• UniProt Gene Name: BRCA1
• UniProt Synonym Gene Names: RNF53

NCBI Description

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]

Uniprot Description

E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks. Component of the BRCA1-RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8. Acts as a transcriptional activator (PubMed:20160719).