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Testing Data

anti-Human AKAP14 Antibody | anti-AKAP14 antibody

AKAP14 Antibody

Gene Names
AKAP14; AKAP28; PRKA14
Reactivity
Human
Applications
Western Blot, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
AKAP14; AKAP14 Antibody; A-kinase anchor protein 14; A-kinase anchor protein 28 kDa; AKAP28; anti-AKAP14 antibody
Ordering
For Research Use Only!
Reactivity
Human
Specificity
AKAP14 antibody detects endogenous levels of total AKAP14 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
137
Applicable Applications for anti-AKAP14 antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:5000
Immunogen
The antiserum was produced against synthesized peptide derived from interinal of human AKAP14.
Preparation and Storage
Store at-20 degree C for 1 year.

Testing Data

Testing Data

Western Blot (WB)

(Peptide-+ Western blot analysis of extracts from Jurkat cells, K562 cells and HUVEC cells, using AKAP14 antibody. Immunofluorescence analysis of HeLa cells, using AKAP14 antibody.)

Western Blot (WB) (Peptide-+ Western blot analysis of extracts from Jurkat cells, K562 cells and HUVEC cells, using AKAP14 antibody. Immunofluorescence analysis of HeLa cells, using AKAP14 antibody.)
References
Kultgen P.L., Mol. Biol. Cell 13:4156-4166(2002). The MGC Project Team, Genome Res. 14:2121-2127(2004).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,187 Da
NCBI Official Full Name
A-kinase anchor protein 14 isoform b
NCBI Official Synonym Full Names
A-kinase anchoring protein 14
NCBI Official Symbol
AKAP14
NCBI Official Synonym Symbols
AKAP28; PRKA14
NCBI Protein Information
A-kinase anchor protein 14
UniProt Protein Name
A-kinase anchor protein 14
Protein Family
UniProt Gene Name
AKAP14
UniProt Synonym Gene Names
AKAP28; AKAP-14; AKAP 28; PRKA14

NCBI Description

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The protein anchors PKA in ciliary axonemes and, in this way, may play a role in regulating ciliary beat frequency. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Binds to type II regulatory subunits of protein kinase A and anchors/targets them.

Research Articles on AKAP14

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Product Notes

The AKAP14 akap14 (Catalog #AAA853484) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The AKAP14 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AKAP14 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000. Researchers should empirically determine the suitability of the AKAP14 akap14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKAP14, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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