Immunohistochemistry (IHC)
(Biotinylated (0.01ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
anti-Human ACPP/PAP Antibody | anti-ACPP antibody
Goat anti-ACPP/PAP (aa269-282), Biotinylated Antibody
Gene Names
ACPP; ACP3; 5'-NT; ACP-3
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
ACPP/PAP; Goat anti-ACPP/PAP (aa269-282); Biotinylated Antibody; Goat Anti-ACPP/PAP Antibody; ACPP; acid phosphatase; prostate; ACP-3; ACP3; PAP; OTTHUMP00000216107; prostatic acid phosphatase; prostatic acid phosphotase; anti-ACPP antibody
Reactivity
Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
418
Applicable Applications for anti-ACPP antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Pep-ELISA: antibody detection limit dilution 1:32000.
WB: Approx 50kDa band observed in Human Prostate lysates. Recommended concentration: 0.01-0.03ug/ml.
WB: Approx 50kDa band observed in Human Prostate lysates. Recommended concentration: 0.01-0.03ug/ml.
Immunogen Sequence
Peptide with sequence C-NHMKRATQIPSYKK., from the internal region of the protein sequence according to NP_001127666.1; NP_001090.2.
Peptide Sequence + C
C-NHMKRATQIPSYKK.
Epitope
Internal Region
Note
This antibody is expected to recognize both reported isoforms (NP_001127666.1; NP_001090.2).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Immunohistochemistry (IHC)
(Biotinylated (0.01ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
(Biotinylated (0.01ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48.3kDa
NCBI Official Full Name
prostatic acid phosphatase isoform TM-PAP
NCBI Official Synonym Full Names
acid phosphatase, prostate
NCBI Official Symbol
ACPP
NCBI Official Synonym Symbols
ACP3; 5'-NT; ACP-3
NCBI Protein Information
prostatic acid phosphatase
UniProt Protein Name
Prostatic acid phosphatase
Protein Family
UniProt Gene Name
ACPP
UniProt Synonym Gene Names
PAP; 5'-NT; TMPase
NCBI Description
This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq, Sep 2008]
Uniprot Description
A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma.
Research Articles on ACPP
Similar Products
Product Notes
The ACPP acpp (Catalog #AAA423752) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ACPP/PAP (aa269-282), Biotinylated Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ACPP/PAP can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Pep-ELISA: antibody detection limit dilution 1:32000. WB: Approx 50kDa band observed in Human Prostate lysates. Recommended concentration: 0.01-0.03ug/ml. Researchers should empirically determine the suitability of the ACPP acpp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACPP/PAP, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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