Western Blot (WB)
()
WNT1 active protein
WNT1 protein
Purity
> 98% pureWNT1 protein was purified using a a proprietary chromatographic technique.
Synonyms
WNT1; WNT1 protein; WNT 1; Wnt 1 protein; WNT-1; WNT 1 protein; Wnt-1 protein; INT-1 protein; WNT-1 protein; WNT1 active protein
Host
Human
Purity/Purification
> 98% pure
WNT1 protein was purified using a a proprietary chromatographic technique.
WNT1 protein was purified using a a proprietary chromatographic technique.
Form/Format
Supplied in lyophilized form with no additives
Sequence Length
258
Protein Type
Recombinant
Biological Significance
Wnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, beta-catenin. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers.
Bioactivity
The ED50 was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells The expected ED50 for this effect is 15-25 ng/ml in the presence of 200 ng/ml of human BMP-2
Endotoxin Levels
<0.1 ng/ug
Expression System
E Coli
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
()
Western Blot (WB)
()
Related Product Information for WNT1 active protein
Purified recombinant Human WNT1 protein
Product Categories/Family for WNT1 active protein
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
38.4 kDa
NCBI Official Full Name
Wnt1, partial
UniProt Protein Name
Protein Wnt
Protein Family
UniProt Gene Name
Wnt1
UniProt Entry Name
C4P689_OCTBM
Uniprot Description
Ligand for members of the frizzled family of seven transmembrane receptors.
Similar Products
Product Notes
The WNT1 wnt1 (Catalog #AAA5304116) is an Active Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "WNT1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
